Thulium- and Erbium-Doped Nanoparticles with Poly(acrylic acid) Coating for Upconversion Cross-Correlation Spectroscopy-based Sandwich Immunoassays in Plasma
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Thulium- and Erbium-Doped Nanoparticles with Poly(acrylic acid) Coating for Upconversion Cross-Correlation Spectroscopy-based Sandwich Immunoassays in Plasma. / Lahtinen, Satu; Liisberg, Mikkel Baldtzer; Raiko, Kirsti; Krause, Stefan; Soukka, Tero; Vosch, Tom.
I: ACS Applied Nano Materials, Bind 4, Nr. 1, 2021, s. 432-440.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Thulium- and Erbium-Doped Nanoparticles with Poly(acrylic acid) Coating for Upconversion Cross-Correlation Spectroscopy-based Sandwich Immunoassays in Plasma
AU - Lahtinen, Satu
AU - Liisberg, Mikkel Baldtzer
AU - Raiko, Kirsti
AU - Krause, Stefan
AU - Soukka, Tero
AU - Vosch, Tom
PY - 2021
Y1 - 2021
N2 - Fluorescence correlation spectroscopy based on upconverting nano-particles (UCNPs) can detect single particles in strongly autofluorescent samples, such as plasma. Nanoparticle aggregation, however, is a problem in correlation spectroscopy measurements, and biological media are known to induce aggregation. Here we present an improved UCNP surface chemistry and use these UCNPs in an upconversion cross-correlation spectroscopy (UCCS)-based homogeneous immunoassay for thyroid-stimulating hormone, where the simultaneous emission of green- (NaYF4 :Yb3+,Er3+ ) and blue- (NaYF4 :Yb3+,Tm3+) emitting UCNPs is detected when they are bound together by the analyte. The improved coating suppresses UCNP aggregation, even in plasma samples. Additionally, increasing the Tm doping of blue-emitting UCNPs enhances their brightness at a high excitation intensity and also results in a significantly shorter luminescence decay time, which improves the probability of detecting coincident blue and green emission from the bound UCNPs in the UCCS immunoassay.
AB - Fluorescence correlation spectroscopy based on upconverting nano-particles (UCNPs) can detect single particles in strongly autofluorescent samples, such as plasma. Nanoparticle aggregation, however, is a problem in correlation spectroscopy measurements, and biological media are known to induce aggregation. Here we present an improved UCNP surface chemistry and use these UCNPs in an upconversion cross-correlation spectroscopy (UCCS)-based homogeneous immunoassay for thyroid-stimulating hormone, where the simultaneous emission of green- (NaYF4 :Yb3+,Er3+ ) and blue- (NaYF4 :Yb3+,Tm3+) emitting UCNPs is detected when they are bound together by the analyte. The improved coating suppresses UCNP aggregation, even in plasma samples. Additionally, increasing the Tm doping of blue-emitting UCNPs enhances their brightness at a high excitation intensity and also results in a significantly shorter luminescence decay time, which improves the probability of detecting coincident blue and green emission from the bound UCNPs in the UCCS immunoassay.
KW - reduced aggregation
KW - high lanthanide doping
KW - thyroid stimulating hormone
KW - single-molecule detection
KW - correlation spectroscopy
KW - FLUORESCENCE CORRELATION SPECTROSCOPY
KW - PHOTON-UPCONVERTING NANOPARTICLES
KW - SINGLE-MOLECULE DETECTION
KW - SENSITIVITY
KW - PROVIDE
KW - ASSAY
KW - TIME
U2 - 10.1021/acsanm.0c02770
DO - 10.1021/acsanm.0c02770
M3 - Journal article
VL - 4
SP - 432
EP - 440
JO - ACS Applied Nano Materials
JF - ACS Applied Nano Materials
SN - 2574-0970
IS - 1
ER -
ID: 286635513