The transcriptionally active regions in the genome of Bacillus subtilis
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The majority of all genes have so far been identified and annotated systematically through in silico gene finding. Here we report the finding of 3662 strand-specific transcriptionally active regions (TARs) in the genome of Bacillus subtilis by the use of tiling arrays. We have measured the genome-wide expression during mid-exponential growth on rich (LB) and minimal (M9) medium. The identified TARs account for 77.3% of the genes as they are currently annotated and additionally we find 84 putative non-coding RNAs (ncRNAs) and 127 antisense transcripts. One ncRNA, ncr22, is predicted to act as a translational control on cstA and an antisense transcript was observed opposite the housekeeping sigma factor sigA. Through this work we have discovered a long conserved 3' untranslated region (UTR) in a group of membrane-associated genes that is predicted to fold into a large and highly stable secondary structure. One of the genes having this tail is efeN, which encodes a target of the twin-arginine translocase (Tat) protein translocation system.
Originalsprog | Engelsk |
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Tidsskrift | Molecular Microbiology |
Vol/bind | 73 |
Udgave nummer | 6 |
Sider (fra-til) | 1043-57 |
Antal sider | 15 |
ISSN | 0950-382X |
DOI | |
Status | Udgivet - sep. 2009 |
Eksternt udgivet | Ja |
ID: 210773333