The transcriptionally active regions in the genome of Bacillus subtilis

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Standard

The transcriptionally active regions in the genome of Bacillus subtilis. / Rasmussen, Simon; Nielsen, Henrik Bjørn; Jarmer, Hanne.

I: Molecular Microbiology, Bind 73, Nr. 6, 09.2009, s. 1043-57.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Rasmussen, S, Nielsen, HB & Jarmer, H 2009, 'The transcriptionally active regions in the genome of Bacillus subtilis', Molecular Microbiology, bind 73, nr. 6, s. 1043-57. https://doi.org/10.1111/j.1365-2958.2009.06830.x

APA

Rasmussen, S., Nielsen, H. B., & Jarmer, H. (2009). The transcriptionally active regions in the genome of Bacillus subtilis. Molecular Microbiology, 73(6), 1043-57. https://doi.org/10.1111/j.1365-2958.2009.06830.x

Vancouver

Rasmussen S, Nielsen HB, Jarmer H. The transcriptionally active regions in the genome of Bacillus subtilis. Molecular Microbiology. 2009 sep.;73(6):1043-57. https://doi.org/10.1111/j.1365-2958.2009.06830.x

Author

Rasmussen, Simon ; Nielsen, Henrik Bjørn ; Jarmer, Hanne. / The transcriptionally active regions in the genome of Bacillus subtilis. I: Molecular Microbiology. 2009 ; Bind 73, Nr. 6. s. 1043-57.

Bibtex

@article{09e3407112eb472583924b7ac3d799a0,
title = "The transcriptionally active regions in the genome of Bacillus subtilis",
abstract = "The majority of all genes have so far been identified and annotated systematically through in silico gene finding. Here we report the finding of 3662 strand-specific transcriptionally active regions (TARs) in the genome of Bacillus subtilis by the use of tiling arrays. We have measured the genome-wide expression during mid-exponential growth on rich (LB) and minimal (M9) medium. The identified TARs account for 77.3% of the genes as they are currently annotated and additionally we find 84 putative non-coding RNAs (ncRNAs) and 127 antisense transcripts. One ncRNA, ncr22, is predicted to act as a translational control on cstA and an antisense transcript was observed opposite the housekeeping sigma factor sigA. Through this work we have discovered a long conserved 3' untranslated region (UTR) in a group of membrane-associated genes that is predicted to fold into a large and highly stable secondary structure. One of the genes having this tail is efeN, which encodes a target of the twin-arginine translocase (Tat) protein translocation system.",
keywords = "Bacillus subtilis/genetics, Base Sequence, Culture Media/chemistry, Gene Expression Profiling/methods, Models, Molecular, Molecular Sequence Data, Nucleic Acid Conformation, RNA, Antisense/biosynthesis, RNA, Untranslated/biosynthesis, Transcription, Genetic",
author = "Simon Rasmussen and Nielsen, {Henrik Bj{\o}rn} and Hanne Jarmer",
year = "2009",
month = sep,
doi = "10.1111/j.1365-2958.2009.06830.x",
language = "English",
volume = "73",
pages = "1043--57",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley-Blackwell",
number = "6",

}

RIS

TY - JOUR

T1 - The transcriptionally active regions in the genome of Bacillus subtilis

AU - Rasmussen, Simon

AU - Nielsen, Henrik Bjørn

AU - Jarmer, Hanne

PY - 2009/9

Y1 - 2009/9

N2 - The majority of all genes have so far been identified and annotated systematically through in silico gene finding. Here we report the finding of 3662 strand-specific transcriptionally active regions (TARs) in the genome of Bacillus subtilis by the use of tiling arrays. We have measured the genome-wide expression during mid-exponential growth on rich (LB) and minimal (M9) medium. The identified TARs account for 77.3% of the genes as they are currently annotated and additionally we find 84 putative non-coding RNAs (ncRNAs) and 127 antisense transcripts. One ncRNA, ncr22, is predicted to act as a translational control on cstA and an antisense transcript was observed opposite the housekeeping sigma factor sigA. Through this work we have discovered a long conserved 3' untranslated region (UTR) in a group of membrane-associated genes that is predicted to fold into a large and highly stable secondary structure. One of the genes having this tail is efeN, which encodes a target of the twin-arginine translocase (Tat) protein translocation system.

AB - The majority of all genes have so far been identified and annotated systematically through in silico gene finding. Here we report the finding of 3662 strand-specific transcriptionally active regions (TARs) in the genome of Bacillus subtilis by the use of tiling arrays. We have measured the genome-wide expression during mid-exponential growth on rich (LB) and minimal (M9) medium. The identified TARs account for 77.3% of the genes as they are currently annotated and additionally we find 84 putative non-coding RNAs (ncRNAs) and 127 antisense transcripts. One ncRNA, ncr22, is predicted to act as a translational control on cstA and an antisense transcript was observed opposite the housekeeping sigma factor sigA. Through this work we have discovered a long conserved 3' untranslated region (UTR) in a group of membrane-associated genes that is predicted to fold into a large and highly stable secondary structure. One of the genes having this tail is efeN, which encodes a target of the twin-arginine translocase (Tat) protein translocation system.

KW - Bacillus subtilis/genetics

KW - Base Sequence

KW - Culture Media/chemistry

KW - Gene Expression Profiling/methods

KW - Models, Molecular

KW - Molecular Sequence Data

KW - Nucleic Acid Conformation

KW - RNA, Antisense/biosynthesis

KW - RNA, Untranslated/biosynthesis

KW - Transcription, Genetic

U2 - 10.1111/j.1365-2958.2009.06830.x

DO - 10.1111/j.1365-2958.2009.06830.x

M3 - Journal article

C2 - 19682248

VL - 73

SP - 1043

EP - 1057

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 6

ER -

ID: 210773333