The Smc5-Smc6 complex and SUMO modification of Rad52 regulates recombinational repair at the ribosomal gene locus.
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The Smc5-Smc6 complex and SUMO modification of Rad52 regulates recombinational repair at the ribosomal gene locus. / Torres-Rosell, Jordi; Sunjevaric, Ivana; De Piccoli, Giacomo; Sacher, Meik; Eckert-Boulet, Nadine; Reid, Robert; Jentsch, Stefan; Rothstein, Rodney; Aragón, Luis; Lisby, Michael.
I: Nature Cell Biology, Bind 9, Nr. 8, 2007, s. 923-31.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The Smc5-Smc6 complex and SUMO modification of Rad52 regulates recombinational repair at the ribosomal gene locus.
AU - Torres-Rosell, Jordi
AU - Sunjevaric, Ivana
AU - De Piccoli, Giacomo
AU - Sacher, Meik
AU - Eckert-Boulet, Nadine
AU - Reid, Robert
AU - Jentsch, Stefan
AU - Rothstein, Rodney
AU - Aragón, Luis
AU - Lisby, Michael
N1 - Keywords: Cell Cycle Proteins; Cell Nucleolus; DNA Damage; DNA Repair; DNA, Ribosomal; Rad52 DNA Repair and Recombination Protein; Recombination, Genetic; Ribosomes; SUMO-1 Protein; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins
PY - 2007
Y1 - 2007
N2 - Homologous recombination (HR) is crucial for maintaining genome integrity by repairing DNA double-strand breaks (DSBs) and rescuing collapsed replication forks. In contrast, uncontrolled HR can lead to chromosome translocations, loss of heterozygosity, and deletion of repetitive sequences. Controlled HR is particularly important for the preservation of repetitive sequences of the ribosomal gene (rDNA) cluster. Here we show that recombinational repair of a DSB in rDNA in Saccharomyces cerevisiae involves the transient relocalization of the lesion to associate with the recombination machinery at an extranucleolar site. The nucleolar exclusion of Rad52 recombination foci entails Mre11 and Smc5-Smc6 complexes and depends on Rad52 SUMO (small ubiquitin-related modifier) modification. Remarkably, mutations that abrogate these activities result in the formation of Rad52 foci within the nucleolus and cause rDNA hyperrecombination and the excision of extrachromosomal rDNA circles. Our study also suggests a key role of sumoylation for nucleolar dynamics, perhaps in the compartmentalization of nuclear activities.
AB - Homologous recombination (HR) is crucial for maintaining genome integrity by repairing DNA double-strand breaks (DSBs) and rescuing collapsed replication forks. In contrast, uncontrolled HR can lead to chromosome translocations, loss of heterozygosity, and deletion of repetitive sequences. Controlled HR is particularly important for the preservation of repetitive sequences of the ribosomal gene (rDNA) cluster. Here we show that recombinational repair of a DSB in rDNA in Saccharomyces cerevisiae involves the transient relocalization of the lesion to associate with the recombination machinery at an extranucleolar site. The nucleolar exclusion of Rad52 recombination foci entails Mre11 and Smc5-Smc6 complexes and depends on Rad52 SUMO (small ubiquitin-related modifier) modification. Remarkably, mutations that abrogate these activities result in the formation of Rad52 foci within the nucleolus and cause rDNA hyperrecombination and the excision of extrachromosomal rDNA circles. Our study also suggests a key role of sumoylation for nucleolar dynamics, perhaps in the compartmentalization of nuclear activities.
U2 - 10.1038/ncb1619
DO - 10.1038/ncb1619
M3 - Journal article
C2 - 17643116
VL - 9
SP - 923
EP - 931
JO - Nature Cell Biology
JF - Nature Cell Biology
SN - 1465-7392
IS - 8
ER -
ID: 6567376