The shift from early to late types of ribosomes in zebrafish development involves changes at a subset of rRNA 2'-O-Me sites
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The shift from early to late types of ribosomes in zebrafish development involves changes at a subset of rRNA 2'-O-Me sites. / Ramachandran, Sowmya; Krogh, Nicolai; Jørgensen, Tor Erik; Johansen, Steinar Daae; Nielsen, Henrik; Babiak, Igor.
I: R N A, Bind 26, Nr. 12, 12.2020, s. 1919-1934.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The shift from early to late types of ribosomes in zebrafish development involves changes at a subset of rRNA 2'-O-Me sites
AU - Ramachandran, Sowmya
AU - Krogh, Nicolai
AU - Jørgensen, Tor Erik
AU - Johansen, Steinar Daae
AU - Nielsen, Henrik
AU - Babiak, Igor
N1 - © 2020 Ramachandran et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.
PY - 2020/12
Y1 - 2020/12
N2 - During zebrafish development, an early type of rRNA is gradually replaced by a late type that is substantially different in sequence. We applied RiboMeth-seq to rRNA from developmental stages for profiling of 2'-O-Me, to learn if changes in methylation pattern were a component of the shift. We compiled a catalog of 2'-O-Me sites and cognate box C/D guide RNAs comprising 98 high-confidence sites, including 10 sites that were not known from other vertebrates, one of which was specific to late-type rRNA. We identified a subset of sites that changed in methylation status during development and found that some of these could be explained by availability of their cognate SNORDs. Sites that changed during development were enriched in the novel sites revealed in zebrafish. We propose that the early type of rRNA is a specialized form and that its structure and ribose methylation pattern may be an adaptation to features of development, including translation of specific maternal mRNAs.
AB - During zebrafish development, an early type of rRNA is gradually replaced by a late type that is substantially different in sequence. We applied RiboMeth-seq to rRNA from developmental stages for profiling of 2'-O-Me, to learn if changes in methylation pattern were a component of the shift. We compiled a catalog of 2'-O-Me sites and cognate box C/D guide RNAs comprising 98 high-confidence sites, including 10 sites that were not known from other vertebrates, one of which was specific to late-type rRNA. We identified a subset of sites that changed in methylation status during development and found that some of these could be explained by availability of their cognate SNORDs. Sites that changed during development were enriched in the novel sites revealed in zebrafish. We propose that the early type of rRNA is a specialized form and that its structure and ribose methylation pattern may be an adaptation to features of development, including translation of specific maternal mRNAs.
U2 - 10.1261/rna.076760.120
DO - 10.1261/rna.076760.120
M3 - Journal article
C2 - 32912962
VL - 26
SP - 1919
EP - 1934
JO - RNA
JF - RNA
SN - 1355-8382
IS - 12
ER -
ID: 251944900