The Rho-GTPase cdc42 regulates neural progenitor fate at the apical surface.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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The Rho-GTPase cdc42 regulates neural progenitor fate at the apical surface. / Cappello, Silvia; Attardo, Alessio; Wu, Xunwei; Iwasato, Takuji; Itohara, Shigeyoshi; Wilsch-Bräuninger, Michaela; Eilken, Hanna M; Rieger, Michael A; Schroeder, Timm T; Huttner, Wieland B; Brakebusch, Cord; Götz, Magdalena.
I: Nature Neuroscience, Bind 9, Nr. 9, 2006, s. 1099-107.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The Rho-GTPase cdc42 regulates neural progenitor fate at the apical surface.
AU - Cappello, Silvia
AU - Attardo, Alessio
AU - Wu, Xunwei
AU - Iwasato, Takuji
AU - Itohara, Shigeyoshi
AU - Wilsch-Bräuninger, Michaela
AU - Eilken, Hanna M
AU - Rieger, Michael A
AU - Schroeder, Timm T
AU - Huttner, Wieland B
AU - Brakebusch, Cord
AU - Götz, Magdalena
N1 - Keywords: Adherens Junctions; Animals; Cell Cycle; Cell Division; Cell Lineage; Female; Gene Deletion; Immunohistochemistry; In Situ Hybridization; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Mitosis; Nerve Tissue; Neuroepithelial Cells; Stem Cells; Telencephalon; cdc42 GTP-Binding Protein
PY - 2006
Y1 - 2006
N2 - Stem cell persistence into adulthood requires self-renewal from early developmental stages. In the developing mouse brain, only apical progenitors located at the ventricle are self-renewing, whereas basal progenitors gradually deplete. However, nothing is known about the mechanisms regulating the fundamental difference between these progenitors. Here we show that the conditional deletion of the small Rho-GTPase cdc42 at different stages of neurogenesis in mouse telencephalon results in an immediate increase in basal mitoses. Whereas cdc42-deficient progenitors have normal cell cycle length, orientation of cell division and basement membrane contact, the apical location of the Par complex and adherens junctions are gradually lost, leading to an increasing failure of apically directed interkinetic nuclear migration. These cells then undergo mitoses at basal positions and acquire the fate of basal progenitors. Thus, cdc42 has a crucial role at the apical pole of progenitors, thereby regulating the position of mitoses and cell fate.
AB - Stem cell persistence into adulthood requires self-renewal from early developmental stages. In the developing mouse brain, only apical progenitors located at the ventricle are self-renewing, whereas basal progenitors gradually deplete. However, nothing is known about the mechanisms regulating the fundamental difference between these progenitors. Here we show that the conditional deletion of the small Rho-GTPase cdc42 at different stages of neurogenesis in mouse telencephalon results in an immediate increase in basal mitoses. Whereas cdc42-deficient progenitors have normal cell cycle length, orientation of cell division and basement membrane contact, the apical location of the Par complex and adherens junctions are gradually lost, leading to an increasing failure of apically directed interkinetic nuclear migration. These cells then undergo mitoses at basal positions and acquire the fate of basal progenitors. Thus, cdc42 has a crucial role at the apical pole of progenitors, thereby regulating the position of mitoses and cell fate.
U2 - 10.1038/nn1744
DO - 10.1038/nn1744
M3 - Journal article
C2 - 16892058
VL - 9
SP - 1099
EP - 1107
JO - Nature Neuroscience
JF - Nature Neuroscience
SN - 1097-6256
IS - 9
ER -
ID: 5140988