The immunoglobulin superfamily member CD200R identifies cells involved in type 2 immune responses
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
BACKGROUND: The pathology of allergic diseases involves type 2 immune cells, such as Th2, ILC2, and basophils exerting their effect by production of IL-4, IL-5, and IL-13. However, surface receptors that are specifically expressed on type 2 immune cells are less well documented. The aim of this investigation was to identify surface markers associated with type 2 inflammation.
METHODS: Naïve human CD4(+) T-cells were short-term activated in the presence or absence of IL-4, and analysed for expression of >300 cell-surface proteins. Ex vivo isolated PBMCs from peanut and non-allergic allergic subjects, were stimulated (14-16h) with peanut extract to detect peanut-specific CD4(+) CD154(+) T-cells. Biopsies were obtained for transcriptomic analysis from healthy controls and patients with extrinsic or intrinsic atopic dermatitis and psoriasis.
RESULTS: Expression analysis of >300 surface proteins enabled identification of IL-4 up-regulated surface proteins, such as CD90, CD108, CD109, and CD200R (CD200R1). Additional analysis of in vitro differentiated Th0, Th1, and Th2 cultures, identified CD200R as up-regulated on Th2 cells. From ex vivo isolated PBMCs, we found high expression of CD200R on Th2 and ILC2 cells and basophils. In peanut-allergic subjects the peanut-specific Th2 (CD154(+) CRTh2(+) ) cells expressed more CD200R than the non-allergen specific Th2 (CD154(-) CRTh2(+) ) cells. Moreover, co-staining of CD161 and CD200R identified peanut-specific highly differentiated IL-4(+) IL-5(+) Th2 cells. Finally, transcriptomic analysis revealed up-regulation of CD200R in lesional skin from subjects with an extrinsic atopic dermatitis phenotype compared to healthy skin.
CONCLUSION: These results indicate that CD200R expression strongly correlates with Th2 pathology; though, the mechanism is as yet elusive. This article is protected by copyright. All rights reserved.
Originalsprog | Engelsk |
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Tidsskrift | Allergy |
Vol/bind | 72 |
Udgave nummer | 7 |
Sider (fra-til) | 1081-1090 |
ISSN | 0105-4538 |
DOI | |
Status | Udgivet - jul. 2017 |
ID: 172699927