The DNA damage response at eroded telomeres and tethering to the nuclear pore complex
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The DNA damage response at eroded telomeres and tethering to the nuclear pore complex. / Khadaroo, Basheer; Teixeira, M Teresa; Luciano, Pierre; Eckert-Boulet, Nadine; Germann, Susanne M; Simon, Marie Noelle; Gallina, Irene; Abdallah, Pauline; Gilson, Eric; Géli, Vincent; Lisby, Michael.
I: Nature Cell Biology, Bind 11, Nr. 8, 2009, s. 980-7.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The DNA damage response at eroded telomeres and tethering to the nuclear pore complex
AU - Khadaroo, Basheer
AU - Teixeira, M Teresa
AU - Luciano, Pierre
AU - Eckert-Boulet, Nadine
AU - Germann, Susanne M
AU - Simon, Marie Noelle
AU - Gallina, Irene
AU - Abdallah, Pauline
AU - Gilson, Eric
AU - Géli, Vincent
AU - Lisby, Michael
N1 - Keywords: Adaptor Proteins, Signal Transducing; Cell Cycle Proteins; Chromatin Immunoprecipitation; DNA Damage; DNA Repair; DNA, Single-Stranded; G2 Phase; Haploidy; Luminescent Proteins; Microscopy, Fluorescence; Mutation; Nuclear Pore; Nuclear Pore Complex Proteins; Rad52 DNA Repair and Recombination Protein; Recombinant Fusion Proteins; Replication Protein A; S Phase; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Telomerase; Telomere; Telomere-Binding Proteins
PY - 2009
Y1 - 2009
N2 - The ends of linear eukaryotic chromosomes are protected by telomeres, which serve to ensure proper chromosome replication and to prevent spurious recombination at chromosome ends. In this study, we show by single cell analysis that in the absence of telomerase, a single short telomere is sufficient to induce the recruitment of checkpoint and recombination proteins. Notably, a DNA damage response at eroded telomeres starts many generations before senescence and is characterized by the recruitment of Cdc13 (cell division cycle 13), replication protein A, DNA damage checkpoint proteins and the DNA repair protein Rad52 into a single focus. Moreover, we show that eroded telomeres, although remaining at the nuclear periphery, move to the nuclear pore complex. Our results link the DNA damage response at eroded telomeres to changes in subnuclear localization and suggest the existence of collapsed replication forks at eroded telomeres.
AB - The ends of linear eukaryotic chromosomes are protected by telomeres, which serve to ensure proper chromosome replication and to prevent spurious recombination at chromosome ends. In this study, we show by single cell analysis that in the absence of telomerase, a single short telomere is sufficient to induce the recruitment of checkpoint and recombination proteins. Notably, a DNA damage response at eroded telomeres starts many generations before senescence and is characterized by the recruitment of Cdc13 (cell division cycle 13), replication protein A, DNA damage checkpoint proteins and the DNA repair protein Rad52 into a single focus. Moreover, we show that eroded telomeres, although remaining at the nuclear periphery, move to the nuclear pore complex. Our results link the DNA damage response at eroded telomeres to changes in subnuclear localization and suggest the existence of collapsed replication forks at eroded telomeres.
U2 - 10.1038/ncb1910
DO - 10.1038/ncb1910
M3 - Journal article
C2 - 19597487
VL - 11
SP - 980
EP - 987
JO - Nature Cell Biology
JF - Nature Cell Biology
SN - 1465-7392
IS - 8
ER -
ID: 16586157