The action of neutrophil serine proteases on elastin and its precursor
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The action of neutrophil serine proteases on elastin and its precursor. / Heinz, Andrea; Jung, Michael C; Jahreis, Günther; Rusciani, Anthony; Duca, Laurent; Debelle, Laurent; Weiss, Anthony S; Neubert, Reinhard H H; Schmelzer, Christian E H.
I: Biochimie, Bind 94, Nr. 1, 01.2012, s. 192-202.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The action of neutrophil serine proteases on elastin and its precursor
AU - Heinz, Andrea
AU - Jung, Michael C
AU - Jahreis, Günther
AU - Rusciani, Anthony
AU - Duca, Laurent
AU - Debelle, Laurent
AU - Weiss, Anthony S
AU - Neubert, Reinhard H H
AU - Schmelzer, Christian E H
N1 - Copyright © 2011 Elsevier Masson SAS. All rights reserved.
PY - 2012/1
Y1 - 2012/1
N2 - This study aimed to investigate the degradation of the natural substrates tropoelastin and elastin by the neutrophil-derived serine proteases human leukocyte elastase (HLE), proteinase 3 (PR3) and cathepsin G (CG). Focus was placed on determining their cleavage site specificities using mass spectrometric techniques. Moreover, the release of bioactive peptides from elastin by the three proteases was studied. Tropoelastin was comprehensively degraded by all three proteases, whereas less cleavage occurred in mature cross-linked elastin. An analysis of the cleavage site specificities of the three proteases in tropoelastin and elastin revealed that HLE and PR3 similarly tolerate hydrophobic and/or aliphatic amino acids such as Ala, Gly and Val at P(1), which are also preferred by CG. In addition, CG prefers the bulky hydrophobic amino acid Leu and accepts the bulky aromatic amino acids Phe and Tyr. CG shows a strong preference for the charged amino acid Lys at P(1) in tropoelastin, whereas Lys was not identified at P(1) in CG digests of elastin due to extensive cross-linking at Lys residues in mature elastin. All three serine proteases showed a clear preference for Pro at P(2) and P(4)'. With respect to the liberation of potentially bioactive peptides from elastin, the study revealed that all three serine proteases have a similar ability to release bioactive sequences, with CG producing the highest number of these peptides. In bioactivity studies, potentially bioactive peptides that have not been investigated on their bioactivity to date, were tested. Three new bioactive GxxPG motifs were identified; GVYPG, GFGPG and GVLPG.
AB - This study aimed to investigate the degradation of the natural substrates tropoelastin and elastin by the neutrophil-derived serine proteases human leukocyte elastase (HLE), proteinase 3 (PR3) and cathepsin G (CG). Focus was placed on determining their cleavage site specificities using mass spectrometric techniques. Moreover, the release of bioactive peptides from elastin by the three proteases was studied. Tropoelastin was comprehensively degraded by all three proteases, whereas less cleavage occurred in mature cross-linked elastin. An analysis of the cleavage site specificities of the three proteases in tropoelastin and elastin revealed that HLE and PR3 similarly tolerate hydrophobic and/or aliphatic amino acids such as Ala, Gly and Val at P(1), which are also preferred by CG. In addition, CG prefers the bulky hydrophobic amino acid Leu and accepts the bulky aromatic amino acids Phe and Tyr. CG shows a strong preference for the charged amino acid Lys at P(1) in tropoelastin, whereas Lys was not identified at P(1) in CG digests of elastin due to extensive cross-linking at Lys residues in mature elastin. All three serine proteases showed a clear preference for Pro at P(2) and P(4)'. With respect to the liberation of potentially bioactive peptides from elastin, the study revealed that all three serine proteases have a similar ability to release bioactive sequences, with CG producing the highest number of these peptides. In bioactivity studies, potentially bioactive peptides that have not been investigated on their bioactivity to date, were tested. Three new bioactive GxxPG motifs were identified; GVYPG, GFGPG and GVLPG.
KW - Amino Acid Sequence
KW - Chromatography, High Pressure Liquid
KW - Elastin
KW - Enzyme-Linked Immunosorbent Assay
KW - Humans
KW - Leukocyte Elastase
KW - Molecular Sequence Data
KW - Neutrophils
KW - Protein Precursors
KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1016/j.biochi.2011.10.006
DO - 10.1016/j.biochi.2011.10.006
M3 - Journal article
C2 - 22030899
VL - 94
SP - 192
EP - 202
JO - Biochimie
JF - Biochimie
SN - 0300-9084
IS - 1
ER -
ID: 186422271