Tachykinins and tachykinin-receptors in the rat pineal gland
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Tachykinins and tachykinin-receptors in the rat pineal gland. / Mukda, S; Chetsawang, B; Govitrapong, P; Schmidt, P T; Hay-Schmidt, Anders; Møller, M.
I: European Journal of Neuroscience, Bind 21, Nr. 10, 05.2005, s. 2743-51.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Tachykinins and tachykinin-receptors in the rat pineal gland
AU - Mukda, S
AU - Chetsawang, B
AU - Govitrapong, P
AU - Schmidt, P T
AU - Hay-Schmidt, Anders
AU - Møller, M
PY - 2005/5
Y1 - 2005/5
N2 - High-pressure liquid chromatography of extracts of rat pineal glands, followed by radio immunological analysis with antibodies against tachykinins, demonstrated the presence of substance P, neurokinin A and neurokinin B in the superficial rat pineal gland. Immunohistochemistry on perfusion-fixed rat brain sections showed substance P and neurokinin A to be present in nerve fibers located both in the perivascular spaces as well as intraparenchymally between the pinealocytes. After extracting total RNA, followed by reverse transcription and polymerase chain reaction amplification with primers specific for NK1-, NK2- and NK3-receptors, agarose gel analysis of the reaction products showed the presence of mRNA encoding all three neurokinin receptors. Immunohistochemical analysis showed NK1 receptor to be located in the interstitial cells of the gland. This location was confirmed by use of in situ hybridization using radioactively labeled antisense oligonucleotide probes. Double immunohistochemical stainings showed that the NK1-immunoreactive cells were not a part of the macrophages or antigen-presenting cells of the gland. Our study suggests that tachykinins, after release from intrapineal nerve fibers, are involved in an up to now unknown function, different from that of melatonin synthesis.
AB - High-pressure liquid chromatography of extracts of rat pineal glands, followed by radio immunological analysis with antibodies against tachykinins, demonstrated the presence of substance P, neurokinin A and neurokinin B in the superficial rat pineal gland. Immunohistochemistry on perfusion-fixed rat brain sections showed substance P and neurokinin A to be present in nerve fibers located both in the perivascular spaces as well as intraparenchymally between the pinealocytes. After extracting total RNA, followed by reverse transcription and polymerase chain reaction amplification with primers specific for NK1-, NK2- and NK3-receptors, agarose gel analysis of the reaction products showed the presence of mRNA encoding all three neurokinin receptors. Immunohistochemical analysis showed NK1 receptor to be located in the interstitial cells of the gland. This location was confirmed by use of in situ hybridization using radioactively labeled antisense oligonucleotide probes. Double immunohistochemical stainings showed that the NK1-immunoreactive cells were not a part of the macrophages or antigen-presenting cells of the gland. Our study suggests that tachykinins, after release from intrapineal nerve fibers, are involved in an up to now unknown function, different from that of melatonin synthesis.
KW - Animals
KW - DNA Primers
KW - Dendritic Cells
KW - Immunohistochemistry
KW - Macrophages
KW - Male
KW - Neurokinin A
KW - Pineal Gland
KW - Rats
KW - Rats, Wistar
KW - Receptors, Neurokinin-1
KW - Receptors, Neurokinin-3
KW - Receptors, Tachykinin
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Substance P
KW - Tachykinins
U2 - 10.1111/j.1460-9568.2005.04088.x
DO - 10.1111/j.1460-9568.2005.04088.x
M3 - Journal article
C2 - 15926922
VL - 21
SP - 2743
EP - 2751
JO - European Journal of Neuroscience
JF - European Journal of Neuroscience
SN - 0953-816X
IS - 10
ER -
ID: 47928583