Synthesis of a-factor peptide from Saccharomyces cerevisiae and photoactive analogues via Fmoc solid phase methodology
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a-Factor from Saccharomyces cerevisiae is a farnesylated dodecapeptide involved in mating. The molecule binds to a G-protein coupled receptor and hence serves as a simple system for studying the interactions between prenylated molecules and their cognate receptors. Here, we describe the preparation of a-factor and two photoactive analogues via Fmoc solid-phase peptide synthesis using hydrazinobenzoyl AM NovaGel™ resin; the structure of the synthetic a-factor was confirmed by MS-MS analysis and NMR; the structures of the analogues were confirmed by MS-MS analysis. Using a yeast growth arrest assay, the analogues were found to have activity comparable to a-factor itself.
Originalsprog | Engelsk |
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Tidsskrift | Bioorganic & Medicinal Chemistry |
Vol/bind | 19 |
Udgave nummer | 1 |
Sider (fra-til) | 490-7 |
Antal sider | 8 |
ISSN | 0968-0896 |
DOI | |
Status | Udgivet - 1 jan. 2011 |
Eksternt udgivet | Ja |
Bibliografisk note
Copyright © 2010 Elsevier Ltd. All rights reserved.
ID: 329436713