Super-Resolution Ultrasound Imaging Provides Quantification of the Renal Cortical and Medullary Vasculature in Obese Zucker Rats: A Pilot Study
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Super-Resolution Ultrasound Imaging Provides Quantification of the Renal Cortical and Medullary Vasculature in Obese Zucker Rats : A Pilot Study. / Søgaard, Stinne Byrholdt; Andersen, Sofie Bech; Taghavi, Iman; Hoyos, Carlos Armando Villagómez; Christoffersen, Christina; Hansen, Kristoffer Lindskov; Jensen, Jørgen Arendt; Nielsen, Michael Bachmann; Sørensen, Charlotte Mehlin.
I: Diagnostics, Bind 12, Nr. 7, 1626, 07.2022.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Super-Resolution Ultrasound Imaging Provides Quantification of the Renal Cortical and Medullary Vasculature in Obese Zucker Rats
T2 - A Pilot Study
AU - Søgaard, Stinne Byrholdt
AU - Andersen, Sofie Bech
AU - Taghavi, Iman
AU - Hoyos, Carlos Armando Villagómez
AU - Christoffersen, Christina
AU - Hansen, Kristoffer Lindskov
AU - Jensen, Jørgen Arendt
AU - Nielsen, Michael Bachmann
AU - Sørensen, Charlotte Mehlin
N1 - Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/7
Y1 - 2022/7
N2 - Obesity is a risk factor of chronic kidney disease (CKD), leading to alterations in the renal vascular structure. This study tested if renal vascular density and tortuosity was quantifiable in vivo in obese rats using microbubble-based super-resolution ultrasound imaging. The kidneys of two 11-week-old and two 20-week-old male obese Zucker rats were compared with age-matched male lean Zucker rats. The super-resolution ultrasound images were manually divided into inner me-dulla, outer medulla, and cortex, and each area was subdivided into arteries and veins. We quanti-fied vascular density and tortuosity, number of detected microbubbles, and generated tracks. For comparison, we assessed glomerular filtration rate, albumin/creatinine ratio, and renal histology to evaluate CKD. The number of detected microbubbles and generated tracks varied between animals and significantly affected quantification of vessel density. In areas with a comparable number of tracks, density increased in the obese animals, concomitant with a decrease in glomerular filtration rate and an increase in albumin/creatinine ratio, but without any pathology in the histological stain-ing. The results indicate that super-resolution ultrasound imaging can be used to quantify structural alterations in the renal vasculature. Techniques to generate more comparable number of microbub-ble tracks and confirmation of the findings in larger-scale studies are needed.
AB - Obesity is a risk factor of chronic kidney disease (CKD), leading to alterations in the renal vascular structure. This study tested if renal vascular density and tortuosity was quantifiable in vivo in obese rats using microbubble-based super-resolution ultrasound imaging. The kidneys of two 11-week-old and two 20-week-old male obese Zucker rats were compared with age-matched male lean Zucker rats. The super-resolution ultrasound images were manually divided into inner me-dulla, outer medulla, and cortex, and each area was subdivided into arteries and veins. We quanti-fied vascular density and tortuosity, number of detected microbubbles, and generated tracks. For comparison, we assessed glomerular filtration rate, albumin/creatinine ratio, and renal histology to evaluate CKD. The number of detected microbubbles and generated tracks varied between animals and significantly affected quantification of vessel density. In areas with a comparable number of tracks, density increased in the obese animals, concomitant with a decrease in glomerular filtration rate and an increase in albumin/creatinine ratio, but without any pathology in the histological stain-ing. The results indicate that super-resolution ultrasound imaging can be used to quantify structural alterations in the renal vasculature. Techniques to generate more comparable number of microbub-ble tracks and confirmation of the findings in larger-scale studies are needed.
KW - chronic kidney disease
KW - image analysis
KW - metabolic syndrome
KW - nephropathy
KW - obesity
KW - ultrasound localization microscopy
U2 - 10.3390/diagnostics12071626
DO - 10.3390/diagnostics12071626
M3 - Journal article
C2 - 35885531
AN - SCOPUS:85133719218
VL - 12
JO - Diagnostics
JF - Diagnostics
SN - 2075-4418
IS - 7
M1 - 1626
ER -
ID: 331497925