Substoichiometric ribose methylations in spliceosomal snRNAs
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Substoichiometric ribose methylations in spliceosomal snRNAs. / Krogh, Nicolai; Kongsbak-Wismann, Martin; Geisler, Carsten; Nielsen, Henrik.
I: Organic and Biomolecular Chemistry, Bind 15, Nr. 42, 2017, s. 8872-8876.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Substoichiometric ribose methylations in spliceosomal snRNAs
AU - Krogh, Nicolai
AU - Kongsbak-Wismann, Martin
AU - Geisler, Carsten
AU - Nielsen, Henrik
PY - 2017
Y1 - 2017
N2 - Sequencing-based profiling of ribose methylations is a new approach that allows for experiments addressing dynamic changes on a large scale. Here, we apply such a method to spliceosomal snRNAs present in human whole cell RNA. Analysis of solid tissue samples confirmed all previously known sites and demonstrated close to full methylation at almost all sites. Methylation changes were revealed in biological experimental settings, using T cell activation as an example, and in the T cell leukemia model, Jurkat cells. Such changes could impact the dynamics of snRNA interactions during the spliceosome cycle and affect mRNA splicing efficiency and splicing patterns.
AB - Sequencing-based profiling of ribose methylations is a new approach that allows for experiments addressing dynamic changes on a large scale. Here, we apply such a method to spliceosomal snRNAs present in human whole cell RNA. Analysis of solid tissue samples confirmed all previously known sites and demonstrated close to full methylation at almost all sites. Methylation changes were revealed in biological experimental settings, using T cell activation as an example, and in the T cell leukemia model, Jurkat cells. Such changes could impact the dynamics of snRNA interactions during the spliceosome cycle and affect mRNA splicing efficiency and splicing patterns.
U2 - 10.1039/c7ob02317k
DO - 10.1039/c7ob02317k
M3 - Journal article
C2 - 29048444
AN - SCOPUS:85032730824
VL - 15
SP - 8872
EP - 8876
JO - Organic & Biomolecular Chemistry
JF - Organic & Biomolecular Chemistry
SN - 1470-4358
IS - 42
ER -
ID: 185720536