Studying RNA-protein interactions in vivo by RNA immunoprecipitation
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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Studying RNA-protein interactions in vivo by RNA immunoprecipitation. / Selth, Luke A; Close, Pierre; Svejstrup, Jesper Q.
Methods in Molecular Biology: Epigenetics Protocols . Bind 791 Springer Science+Business Media, 2011. s. 253-64.Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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TY - CHAP
T1 - Studying RNA-protein interactions in vivo by RNA immunoprecipitation
AU - Selth, Luke A
AU - Close, Pierre
AU - Svejstrup, Jesper Q
PY - 2011
Y1 - 2011
N2 - The crucial roles played by RNA-binding proteins in all aspects of RNA metabolism, particularly in the regulation of transcription, have become increasingly evident. Moreover, other factors that do not directly interact with RNA molecules can nevertheless function proximally to RNA polymerases and have significant effects on gene expression. RNA immunoprecipitation (RIP) is a powerful technique used to detect direct and indirect interactions between individual proteins and specific RNA molecules in vivo. Here, we describe RIP methods for both yeast and mammalian cells.
AB - The crucial roles played by RNA-binding proteins in all aspects of RNA metabolism, particularly in the regulation of transcription, have become increasingly evident. Moreover, other factors that do not directly interact with RNA molecules can nevertheless function proximally to RNA polymerases and have significant effects on gene expression. RNA immunoprecipitation (RIP) is a powerful technique used to detect direct and indirect interactions between individual proteins and specific RNA molecules in vivo. Here, we describe RIP methods for both yeast and mammalian cells.
KW - Chromatin
KW - Fungal Proteins
KW - HEK293 Cells
KW - Humans
KW - Immunoprecipitation
KW - Protein Binding
KW - RNA, Fungal
KW - Saccharomyces cerevisiae
U2 - 10.1007/978-1-61779-316-5_19
DO - 10.1007/978-1-61779-316-5_19
M3 - Book chapter
C2 - 21913085
VL - 791
SP - 253
EP - 264
BT - Methods in Molecular Biology
PB - Springer Science+Business Media
ER -
ID: 38419149