ST6GalNAc-I controls expression of sialyl-Tn antigen in gastrointestinal tissues

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

ST6GalNAc-I controls expression of sialyl-Tn antigen in gastrointestinal tissues. / Marcos, Nuno T; Bennett, Eric P; Gomes, Joana; Magalhaes, Ana; Gomes, Catarina; David, Leonor; Dar, Imran; Jeanneau, Charlotte; Defrees, Shawn; Krustrup, Dorrit; Vogel, Lotte K; Kure, Elin H; Burchell, Joy; Taylor-Papadimitriou, Joyce; Clausen, Henrik; Mandel, Ulla; Reis, Celso A.

I: Frontiers in Bioscience (Elite Edition), Bind 3, 01.06.2011, s. 1443-55.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Marcos, NT, Bennett, EP, Gomes, J, Magalhaes, A, Gomes, C, David, L, Dar, I, Jeanneau, C, Defrees, S, Krustrup, D, Vogel, LK, Kure, EH, Burchell, J, Taylor-Papadimitriou, J, Clausen, H, Mandel, U & Reis, CA 2011, 'ST6GalNAc-I controls expression of sialyl-Tn antigen in gastrointestinal tissues', Frontiers in Bioscience (Elite Edition), bind 3, s. 1443-55.

APA

Marcos, N. T., Bennett, E. P., Gomes, J., Magalhaes, A., Gomes, C., David, L., Dar, I., Jeanneau, C., Defrees, S., Krustrup, D., Vogel, L. K., Kure, E. H., Burchell, J., Taylor-Papadimitriou, J., Clausen, H., Mandel, U., & Reis, C. A. (2011). ST6GalNAc-I controls expression of sialyl-Tn antigen in gastrointestinal tissues. Frontiers in Bioscience (Elite Edition), 3, 1443-55.

Vancouver

Marcos NT, Bennett EP, Gomes J, Magalhaes A, Gomes C, David L o.a. ST6GalNAc-I controls expression of sialyl-Tn antigen in gastrointestinal tissues. Frontiers in Bioscience (Elite Edition). 2011 jun. 1;3:1443-55.

Author

Marcos, Nuno T ; Bennett, Eric P ; Gomes, Joana ; Magalhaes, Ana ; Gomes, Catarina ; David, Leonor ; Dar, Imran ; Jeanneau, Charlotte ; Defrees, Shawn ; Krustrup, Dorrit ; Vogel, Lotte K ; Kure, Elin H ; Burchell, Joy ; Taylor-Papadimitriou, Joyce ; Clausen, Henrik ; Mandel, Ulla ; Reis, Celso A. / ST6GalNAc-I controls expression of sialyl-Tn antigen in gastrointestinal tissues. I: Frontiers in Bioscience (Elite Edition). 2011 ; Bind 3. s. 1443-55.

Bibtex

@article{324b8fd0d9a0450e9c552144f83b525e,
title = "ST6GalNAc-I controls expression of sialyl-Tn antigen in gastrointestinal tissues",
abstract = "Sialyl-Tn is a simple mucin-type carbohydrate antigen aberrantly expressed in gastrointestinal adenocarcinomas and in the precursor lesion intestinal metaplasia. Sialyl-Tn tumour expression is an independent indicator of poor prognosis. We have previously shown in vitro that ST6GalNAc-I and ST6GalNAc-II sialyltransferases can synthesize sialyl-Tn. The aim of the present study was to establish whether ST6GalNAc-I is the major enzyme responsible for the expression of sialyl-Tn. We used a model of CHO-ldlD cells producing only MUC1-Tn glycoform and showed that ST6GalNAc-I is the key-enzyme leading to sialyl-Tn biosynthesis. We developed novel monoclonal antibodies specific for ST6GalNAc-I and evaluated its expression in gastrointestinal tissues. ST6GalNAc-I was detected in normal colon mucosa co-localized with O-acetylated sialyl-Tn. Expression was largely unaltered in colorectal adenocarcinomas. In contrast, we found that ST6GalNAc-I is weakly expressed in normal gastric mucosa, but over-expressed in intestinal metaplasia, co-localized with sialyl-Tn. In gastric carcinomas ST6GalNAc-I was also associated with sialyl-Tn, but with heterogeneous staining and partial co-localization. Our results showed ST6GalNAc-I as the major enzyme controlling the expression of cancer-associated sialyl-Tn antigen in gastrointestinal tissues.",
author = "Marcos, {Nuno T} and Bennett, {Eric P} and Joana Gomes and Ana Magalhaes and Catarina Gomes and Leonor David and Imran Dar and Charlotte Jeanneau and Shawn Defrees and Dorrit Krustrup and Vogel, {Lotte K} and Kure, {Elin H} and Joy Burchell and Joyce Taylor-Papadimitriou and Henrik Clausen and Ulla Mandel and Reis, {Celso A}",
year = "2011",
month = jun,
day = "1",
language = "English",
volume = "3",
pages = "1443--55",
journal = "Frontiers in Bioscience - Elite",
issn = "1945-0494",
publisher = "Frontiers in Bioscience",

}

RIS

TY - JOUR

T1 - ST6GalNAc-I controls expression of sialyl-Tn antigen in gastrointestinal tissues

AU - Marcos, Nuno T

AU - Bennett, Eric P

AU - Gomes, Joana

AU - Magalhaes, Ana

AU - Gomes, Catarina

AU - David, Leonor

AU - Dar, Imran

AU - Jeanneau, Charlotte

AU - Defrees, Shawn

AU - Krustrup, Dorrit

AU - Vogel, Lotte K

AU - Kure, Elin H

AU - Burchell, Joy

AU - Taylor-Papadimitriou, Joyce

AU - Clausen, Henrik

AU - Mandel, Ulla

AU - Reis, Celso A

PY - 2011/6/1

Y1 - 2011/6/1

N2 - Sialyl-Tn is a simple mucin-type carbohydrate antigen aberrantly expressed in gastrointestinal adenocarcinomas and in the precursor lesion intestinal metaplasia. Sialyl-Tn tumour expression is an independent indicator of poor prognosis. We have previously shown in vitro that ST6GalNAc-I and ST6GalNAc-II sialyltransferases can synthesize sialyl-Tn. The aim of the present study was to establish whether ST6GalNAc-I is the major enzyme responsible for the expression of sialyl-Tn. We used a model of CHO-ldlD cells producing only MUC1-Tn glycoform and showed that ST6GalNAc-I is the key-enzyme leading to sialyl-Tn biosynthesis. We developed novel monoclonal antibodies specific for ST6GalNAc-I and evaluated its expression in gastrointestinal tissues. ST6GalNAc-I was detected in normal colon mucosa co-localized with O-acetylated sialyl-Tn. Expression was largely unaltered in colorectal adenocarcinomas. In contrast, we found that ST6GalNAc-I is weakly expressed in normal gastric mucosa, but over-expressed in intestinal metaplasia, co-localized with sialyl-Tn. In gastric carcinomas ST6GalNAc-I was also associated with sialyl-Tn, but with heterogeneous staining and partial co-localization. Our results showed ST6GalNAc-I as the major enzyme controlling the expression of cancer-associated sialyl-Tn antigen in gastrointestinal tissues.

AB - Sialyl-Tn is a simple mucin-type carbohydrate antigen aberrantly expressed in gastrointestinal adenocarcinomas and in the precursor lesion intestinal metaplasia. Sialyl-Tn tumour expression is an independent indicator of poor prognosis. We have previously shown in vitro that ST6GalNAc-I and ST6GalNAc-II sialyltransferases can synthesize sialyl-Tn. The aim of the present study was to establish whether ST6GalNAc-I is the major enzyme responsible for the expression of sialyl-Tn. We used a model of CHO-ldlD cells producing only MUC1-Tn glycoform and showed that ST6GalNAc-I is the key-enzyme leading to sialyl-Tn biosynthesis. We developed novel monoclonal antibodies specific for ST6GalNAc-I and evaluated its expression in gastrointestinal tissues. ST6GalNAc-I was detected in normal colon mucosa co-localized with O-acetylated sialyl-Tn. Expression was largely unaltered in colorectal adenocarcinomas. In contrast, we found that ST6GalNAc-I is weakly expressed in normal gastric mucosa, but over-expressed in intestinal metaplasia, co-localized with sialyl-Tn. In gastric carcinomas ST6GalNAc-I was also associated with sialyl-Tn, but with heterogeneous staining and partial co-localization. Our results showed ST6GalNAc-I as the major enzyme controlling the expression of cancer-associated sialyl-Tn antigen in gastrointestinal tissues.

M3 - Journal article

C2 - 21622148

VL - 3

SP - 1443

EP - 1455

JO - Frontiers in Bioscience - Elite

JF - Frontiers in Bioscience - Elite

SN - 1945-0494

ER -

ID: 33751119