Small RNA target genes and regulatory connections in the Vibrio cholerae quorum sensing system
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Small RNA target genes and regulatory connections in the Vibrio cholerae quorum sensing system. / Hammer, Brian K; Svenningsen, Sine Lo.
Quorum sensing: Methods and protocols. red. / Kendra P. Rumbaugh. Bind 692 Humana Press, 2011. s. 189-206 (Methods in Molecular Biology).Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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TY - CHAP
T1 - Small RNA target genes and regulatory connections in the Vibrio cholerae quorum sensing system
AU - Hammer, Brian K
AU - Svenningsen, Sine Lo
PY - 2011
Y1 - 2011
N2 - The two-component quorum sensing (QS) system, first described in the marine bacterium Vibrio harveyi and evolutionarily conserved among members of the genus Vibrio, has been best studied in the human pathogen Vibrio cholerae (1, 2). In the V. cholerae QS system, the response to the accumulation of extracellular autoinducers triggers a signaling cascade resulting in the transcription of four small regulatory RNAs (sRNAs). Our results support the model that the QS sRNAs bind to the 5' untranslated region of multiple mRNAs and alter the fate of one in a positive manner and several others in a negative manner. This mechanism ensures the proper timing of the QS response, which includes the expression of traits critical for virulence and for the formation of biofilms (2-6).
AB - The two-component quorum sensing (QS) system, first described in the marine bacterium Vibrio harveyi and evolutionarily conserved among members of the genus Vibrio, has been best studied in the human pathogen Vibrio cholerae (1, 2). In the V. cholerae QS system, the response to the accumulation of extracellular autoinducers triggers a signaling cascade resulting in the transcription of four small regulatory RNAs (sRNAs). Our results support the model that the QS sRNAs bind to the 5' untranslated region of multiple mRNAs and alter the fate of one in a positive manner and several others in a negative manner. This mechanism ensures the proper timing of the QS response, which includes the expression of traits critical for virulence and for the formation of biofilms (2-6).
KW - Base Sequence
KW - Binding Sites
KW - Blotting, Northern
KW - Computational Biology
KW - Genes, Bacterial
KW - Green Fluorescent Proteins
KW - Kinetics
KW - Mutagenesis, Site-Directed
KW - Protein Biosynthesis
KW - Quorum Sensing
KW - RNA Splicing
KW - RNA, Messenger
KW - RNA, Untranslated
KW - Reproducibility of Results
KW - Vibrio cholerae
U2 - 10.1007/978-1-60761-971-0_14
DO - 10.1007/978-1-60761-971-0_14
M3 - Book chapter
C2 - 21031313
SN - 978-1-60761-970-3
VL - 692
T3 - Methods in Molecular Biology
SP - 189
EP - 206
BT - Quorum sensing
A2 - Rumbaugh, Kendra P.
PB - Humana Press
ER -
ID: 43239210