Skin surface Protoporphyrin IX fluorescence is associated with epidermal but not dermal fluorescence intensities
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Skin surface Protoporphyrin IX fluorescence is associated with epidermal but not dermal fluorescence intensities. / Foged, Camilla; Philipsen, Peter Alshede; Wulf, Hans Christian; Haedersdal, Merete; Togsverd-Bo, Katrine.
I: Photodiagnosis and Photodynamic Therapy, Bind 30, 101681, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Skin surface Protoporphyrin IX fluorescence is associated with epidermal but not dermal fluorescence intensities
AU - Foged, Camilla
AU - Philipsen, Peter Alshede
AU - Wulf, Hans Christian
AU - Haedersdal, Merete
AU - Togsverd-Bo, Katrine
PY - 2020
Y1 - 2020
N2 - Background: Photodynamic therapy with Protoporphyrin IX (PpIX) is well-established for keratinocyte dysplasia. PpIX fluorescence can be quantified at the skin surface and within skin layers, but their relation is previously undescribed. The study objective was to assess the relation between skin surface PpIX fluorescence and PpIX fluorescence in epidermis and dermis. Methods: Normal buttocks skin was tape-stripped and incubated with 20 % methyl aminolevulinate cream and 20 % hexyl aminolevulinate cream under occlusion. After 3 h, skin surface PpIX fluorescence photographs were captured and 4 mm punch biopsies sampled. PpIX fluorescence microscopy was measured in regions of interest (ROI) in epidermis and superficial dermis. All PpIX fluorescence intensities were calculated in arbitrary units (AU). Results: Skin surface PpIX fluorescence intensity expressed a positive, linear relation to ROI-epidermis PpIX fluorescence (HAL- and MAL-incubated skin, p < 0.001, r2 = 0.60), but not to PpIX fluorescence intensities in ROI-superficial dermis (p = 0.112). PpIX fluorescence microscopy identified higher fluorescence intensities in ROI-epidermis (median 92 AU) compared to lower fluorescence intensities in ROI-superficial dermis (median 37 AU) (p < 0.001). HAL reached higher median PpIX fluorescence intensities than MAL when measured by skin surface photographs (10230 vs. 5630 AU, p < 0.001), andbyfluorescence microscopy in ROI-epidermis (107 vs. 52.5 AU, p < 0.001) or ROI-superficial dermis (40 vs. 30.5 AU, p = 0.04). Conclusion: The positive linear relation between skin surface PpIX fluorescence photographs and epidermal PpIX fluorescence microscopy indicates that skin surface PpIX fluorescence predominantly derives from epidermis.
AB - Background: Photodynamic therapy with Protoporphyrin IX (PpIX) is well-established for keratinocyte dysplasia. PpIX fluorescence can be quantified at the skin surface and within skin layers, but their relation is previously undescribed. The study objective was to assess the relation between skin surface PpIX fluorescence and PpIX fluorescence in epidermis and dermis. Methods: Normal buttocks skin was tape-stripped and incubated with 20 % methyl aminolevulinate cream and 20 % hexyl aminolevulinate cream under occlusion. After 3 h, skin surface PpIX fluorescence photographs were captured and 4 mm punch biopsies sampled. PpIX fluorescence microscopy was measured in regions of interest (ROI) in epidermis and superficial dermis. All PpIX fluorescence intensities were calculated in arbitrary units (AU). Results: Skin surface PpIX fluorescence intensity expressed a positive, linear relation to ROI-epidermis PpIX fluorescence (HAL- and MAL-incubated skin, p < 0.001, r2 = 0.60), but not to PpIX fluorescence intensities in ROI-superficial dermis (p = 0.112). PpIX fluorescence microscopy identified higher fluorescence intensities in ROI-epidermis (median 92 AU) compared to lower fluorescence intensities in ROI-superficial dermis (median 37 AU) (p < 0.001). HAL reached higher median PpIX fluorescence intensities than MAL when measured by skin surface photographs (10230 vs. 5630 AU, p < 0.001), andbyfluorescence microscopy in ROI-epidermis (107 vs. 52.5 AU, p < 0.001) or ROI-superficial dermis (40 vs. 30.5 AU, p = 0.04). Conclusion: The positive linear relation between skin surface PpIX fluorescence photographs and epidermal PpIX fluorescence microscopy indicates that skin surface PpIX fluorescence predominantly derives from epidermis.
KW - Hexyl aminolevulinate
KW - Methyl aminolevulinate
KW - PpIX fluorescence
KW - PpIX fluorescence microscopy
KW - PpIX fluorescence photographs
U2 - 10.1016/j.pdpdt.2020.101681
DO - 10.1016/j.pdpdt.2020.101681
M3 - Journal article
C2 - 32032781
AN - SCOPUS:85079032700
VL - 30
JO - Photodiagnosis and Photodynamic Therapy
JF - Photodiagnosis and Photodynamic Therapy
SN - 1572-1000
M1 - 101681
ER -
ID: 260990654