Sex- and age-specific reference intervals of 16 steroid metabolites quantified simultaneously by LC-MS/MS in sera from 2458 healthy subjects aged 0 to 77 years
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Sex- and age-specific reference intervals of 16 steroid metabolites quantified simultaneously by LC-MS/MS in sera from 2458 healthy subjects aged 0 to 77 years. / Frederiksen, Hanne; Johannsen, Trine Holm; Andersen, Stine Ehlern; Petersen, Jørgen Holm; Busch, Alexander Siegfried; Ljubicic, Marie Lindhardt; Fischer, Margit Bistrup; Upners, Emmie N.; Hagen, Casper P.; Main, Katharina M.; Aksglaede, Lise; Jørgensen, Niels; Lund Kårhus, Line; Linneberg, Allan; Andersson, Anna Maria; Flück, Christa E.; Juul, Anders.
I: Clinica Chimica Acta, Bind 562, 119852, 2024.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Sex- and age-specific reference intervals of 16 steroid metabolites quantified simultaneously by LC-MS/MS in sera from 2458 healthy subjects aged 0 to 77 years
AU - Frederiksen, Hanne
AU - Johannsen, Trine Holm
AU - Andersen, Stine Ehlern
AU - Petersen, Jørgen Holm
AU - Busch, Alexander Siegfried
AU - Ljubicic, Marie Lindhardt
AU - Fischer, Margit Bistrup
AU - Upners, Emmie N.
AU - Hagen, Casper P.
AU - Main, Katharina M.
AU - Aksglaede, Lise
AU - Jørgensen, Niels
AU - Lund Kårhus, Line
AU - Linneberg, Allan
AU - Andersson, Anna Maria
AU - Flück, Christa E.
AU - Juul, Anders
N1 - Publisher Copyright: © 2024
PY - 2024
Y1 - 2024
N2 - Background: Reference intervals covering the whole life span for all the metabolites in the steroid hormone biosynthesis quantified by sensitive and robust analytical methods are sparse or not existing. Objective: To develop a state-of-the-art LC-MS/MS method for simultaneous quantification of multiple steroid metabolites and to establish detailed sex- and age-specific reference intervals for 16 steroid metabolites. Materials and method: An isotope diluted LC-MS/MS method was developed for simultaneous quantitation of 16 steroid hormones. Serum samples from cross-sectional cohorts of healthy infants, children, adolescents, and adults aged 0.17 months to 77 years (n = 2458) were analysed. Results: With this novel, specific, and sensitive LC-MS/MS method, it was possible to quantify progesterone, 17-hydroxypregnenolone, 17-hydroxyprogesterone, dehydroepiandrosterone sulfate, androstenedione, testosterone, dihydrotestosterone, 11-deoxycorticosterone, corticosterone, 11-deoxycortisol, cortisol, and cortisone in ≥90 % of the samples, while estrone sulfate, aldosterone and dehydroepiandrosterone were quantified in 77 %, 75 % and 60 % of the samples, respectively. 21-deoxycortisol was only detectable in 2.5 % of samples from healthy subjects. Sex- and age-dependent fluctuations observed in minipuberty, puberty and adulthood including the menopausal transition were modelled. This enabled us to establish valid reference intervals from birth to late adult life for both males and females. Conclusion: Detailed sex- and age-specific reference intervals of multiple, simultaneously quantified steroid metabolites by a novel and specific LC-MS/MS method provides a valuable tool for clinical practice and for future research.
AB - Background: Reference intervals covering the whole life span for all the metabolites in the steroid hormone biosynthesis quantified by sensitive and robust analytical methods are sparse or not existing. Objective: To develop a state-of-the-art LC-MS/MS method for simultaneous quantification of multiple steroid metabolites and to establish detailed sex- and age-specific reference intervals for 16 steroid metabolites. Materials and method: An isotope diluted LC-MS/MS method was developed for simultaneous quantitation of 16 steroid hormones. Serum samples from cross-sectional cohorts of healthy infants, children, adolescents, and adults aged 0.17 months to 77 years (n = 2458) were analysed. Results: With this novel, specific, and sensitive LC-MS/MS method, it was possible to quantify progesterone, 17-hydroxypregnenolone, 17-hydroxyprogesterone, dehydroepiandrosterone sulfate, androstenedione, testosterone, dihydrotestosterone, 11-deoxycorticosterone, corticosterone, 11-deoxycortisol, cortisol, and cortisone in ≥90 % of the samples, while estrone sulfate, aldosterone and dehydroepiandrosterone were quantified in 77 %, 75 % and 60 % of the samples, respectively. 21-deoxycortisol was only detectable in 2.5 % of samples from healthy subjects. Sex- and age-dependent fluctuations observed in minipuberty, puberty and adulthood including the menopausal transition were modelled. This enabled us to establish valid reference intervals from birth to late adult life for both males and females. Conclusion: Detailed sex- and age-specific reference intervals of multiple, simultaneously quantified steroid metabolites by a novel and specific LC-MS/MS method provides a valuable tool for clinical practice and for future research.
KW - Infant
KW - LC-MS/MS
KW - Puberty
KW - Reference interval
KW - Sex difference
KW - Steroid hormone
U2 - 10.1016/j.cca.2024.119852
DO - 10.1016/j.cca.2024.119852
M3 - Journal article
C2 - 38977173
AN - SCOPUS:85197766961
VL - 562
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
M1 - 119852
ER -
ID: 398463938