Seroreactivity to E. coli outer membrane protein C antibodies in active inflammatory bowel disease; diagnostic value and correlation with phylogroup B2 E. coli infection
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Seroreactivity to E. coli outer membrane protein C antibodies in active inflammatory bowel disease; diagnostic value and correlation with phylogroup B2 E. coli infection. / Petersen, Andreas Munk; Schou, Christian; Mirsepasi, Hengameh; Engberg, Jørgen; Friis-Møller, Alice; Nordgaard-Lassen, Inge; Wildt, Signe; Krogfelt, Karen Angeliki.
I: Scandinavian Journal of Gastroenterology, Bind 47, Nr. 2, 02.2012, s. 155-61.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Seroreactivity to E. coli outer membrane protein C antibodies in active inflammatory bowel disease; diagnostic value and correlation with phylogroup B2 E. coli infection
AU - Petersen, Andreas Munk
AU - Schou, Christian
AU - Mirsepasi, Hengameh
AU - Engberg, Jørgen
AU - Friis-Møller, Alice
AU - Nordgaard-Lassen, Inge
AU - Wildt, Signe
AU - Krogfelt, Karen Angeliki
PY - 2012/2
Y1 - 2012/2
N2 - BACKGROUND: Several serologic tests, including anti-outer membrane porin C antibody (Omp C), are used for screening and as marker of disease course in inflammatory bowel diseases (IBD). Our aim was to investigate possible differences in Omp C level in patients with active and inactive IBD compared to controls.METHODS: All blood samples were tested for Omp C. Disease activity was evaluated by Harvey Bradshaw Index, Simple Clinical Activity Index and Modified Pouchitis Disease Activity Index.RESULTS: Blood samples were collected from 113 patients and 60 controls. Patients with active IBD did not have a higher level of Omp C than patients in remission. Surprisingly, in patients with active Crohn's disease a significantly lower level of Omp C was found compared with patients with inactive Crohn's disease (p < 0.05). All other groups among patients with IBD did have a significantly higher level of Omp C, compared with controls, including patients with acute gastroenteritis (p < 0.05). Although IBD patients with phylogroup B2 E. coli cultured from their fecal samples, were more likely to have a positive Omp C test (p < 0.05), this could not explain the low Omp C level in the subgroup of patients with active Crohn's disease.CONCLUSIONS: Omp C titer was not raised in patients with active IBD compared with patients in remission. In addition, there was no difference in Omp C level in patients with active Crohn's disease compared with controls. These observations do not support the use of Omp C serology testing, either in disease activity assessment, or in screening for active Crohn's disease.
AB - BACKGROUND: Several serologic tests, including anti-outer membrane porin C antibody (Omp C), are used for screening and as marker of disease course in inflammatory bowel diseases (IBD). Our aim was to investigate possible differences in Omp C level in patients with active and inactive IBD compared to controls.METHODS: All blood samples were tested for Omp C. Disease activity was evaluated by Harvey Bradshaw Index, Simple Clinical Activity Index and Modified Pouchitis Disease Activity Index.RESULTS: Blood samples were collected from 113 patients and 60 controls. Patients with active IBD did not have a higher level of Omp C than patients in remission. Surprisingly, in patients with active Crohn's disease a significantly lower level of Omp C was found compared with patients with inactive Crohn's disease (p < 0.05). All other groups among patients with IBD did have a significantly higher level of Omp C, compared with controls, including patients with acute gastroenteritis (p < 0.05). Although IBD patients with phylogroup B2 E. coli cultured from their fecal samples, were more likely to have a positive Omp C test (p < 0.05), this could not explain the low Omp C level in the subgroup of patients with active Crohn's disease.CONCLUSIONS: Omp C titer was not raised in patients with active IBD compared with patients in remission. In addition, there was no difference in Omp C level in patients with active Crohn's disease compared with controls. These observations do not support the use of Omp C serology testing, either in disease activity assessment, or in screening for active Crohn's disease.
KW - Acute Disease
KW - Adolescent
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Antibodies, Bacterial/blood
KW - Bacterial Outer Membrane Proteins/immunology
KW - Child
KW - Chronic Disease
KW - Colitis, Ulcerative/blood
KW - Colonic Pouches/pathology
KW - Crohn Disease/blood
KW - Diarrhea/blood
KW - Dysentery/blood
KW - Escherichia coli/immunology
KW - Escherichia coli Infections/complications
KW - Escherichia coli Proteins/immunology
KW - Female
KW - Gastroenteritis/blood
KW - Humans
KW - Male
KW - Middle Aged
KW - Porins/immunology
KW - Pouchitis/blood
KW - Sensitivity and Specificity
KW - Serologic Tests
KW - Young Adult
U2 - 10.3109/00365521.2011.639080
DO - 10.3109/00365521.2011.639080
M3 - Journal article
C2 - 22150030
VL - 47
SP - 155
EP - 161
JO - Scandinavian Journal of Gastroenterology
JF - Scandinavian Journal of Gastroenterology
SN - 0036-5521
IS - 2
ER -
ID: 209679364