© 2015 Association for Tertiary Education Management and the LH Martin Institute for Tertiary Education Leadership and Management. We analyse data from the largest survey of university staff in Australia to determine whether bullying and harassment are more common in regional than metropolitan and Go8 universities, and to what extent any differences could be attributed to other factors. While professional staff showed no difference in harassment rates between regional and metropolitan and Go8 universities, academic staff at regional universities reported significantly higher levels of harassment. This probably reflected the labour market and resource context of regional universities. Binary logistic regression indicated that a perceived lack of support for professional development partially explained the effect of regional status on differences in the rates of harassment/bullying across university types. Markers of organisational culture only partially account for differences in the rates of harassment/bullying between university types.