Role of elongator subunit Elp3 in Drosophila melanogaster larval development and immunity
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Role of elongator subunit Elp3 in Drosophila melanogaster larval development and immunity. / Walker, Jane; Kwon, So Yeon; Badenhorst, Paul; East, Phil; McNeill, Helen; Svejstrup, Jesper Q.
I: Genetics (Print), Bind 187, Nr. 4, 2011, s. 1067-75.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Role of elongator subunit Elp3 in Drosophila melanogaster larval development and immunity
AU - Walker, Jane
AU - Kwon, So Yeon
AU - Badenhorst, Paul
AU - East, Phil
AU - McNeill, Helen
AU - Svejstrup, Jesper Q
PY - 2011
Y1 - 2011
N2 - The Elongator complex has been implicated in several cellular processes, including gene expression and tRNA modification. We investigated the biological importance of the Elp3 gene in Drosophila melanogaster. Deletion of Elp3 results in larval lethality at the pupal stage. During early development, larval growth is dramatically impaired, with progression to the third instar delayed for ~24 hr, and pupariation occurring only at day 14 after egg laying. Melanotic nodules appear after 4 days. Microarray analysis shows that stress response genes are induced and ecdysone-induced transcription factors are severely repressed in the mutant. Interestingly, the phenotypes of Elp3 flies are similar to those of flies lacking the domino gene, encoding a SWI/SNF-like ATP-dependent chromatin-remodeling enzyme. Indeed, the gene expression profiles of these mutants are also remarkably similar. Together, these data demonstrate that Drosophila Elp3 is essential for viability, normal development, and hematopoiesis and suggest a functional overlap with the chromatin remodeler Domino.
AB - The Elongator complex has been implicated in several cellular processes, including gene expression and tRNA modification. We investigated the biological importance of the Elp3 gene in Drosophila melanogaster. Deletion of Elp3 results in larval lethality at the pupal stage. During early development, larval growth is dramatically impaired, with progression to the third instar delayed for ~24 hr, and pupariation occurring only at day 14 after egg laying. Melanotic nodules appear after 4 days. Microarray analysis shows that stress response genes are induced and ecdysone-induced transcription factors are severely repressed in the mutant. Interestingly, the phenotypes of Elp3 flies are similar to those of flies lacking the domino gene, encoding a SWI/SNF-like ATP-dependent chromatin-remodeling enzyme. Indeed, the gene expression profiles of these mutants are also remarkably similar. Together, these data demonstrate that Drosophila Elp3 is essential for viability, normal development, and hematopoiesis and suggest a functional overlap with the chromatin remodeler Domino.
KW - Animals
KW - Chromatin
KW - Chromatin Assembly and Disassembly
KW - Drosophila Proteins
KW - Drosophila melanogaster
KW - Ecdysone
KW - Gene Deletion
KW - Gene Expression Profiling
KW - Gene Expression Regulation, Developmental
KW - Genes, Lethal
KW - Histone Acetyltransferases
KW - Larva
KW - Linear Models
KW - Nerve Tissue Proteins
KW - Oligonucleotide Array Sequence Analysis
KW - Pupa
KW - Transcription Factors
U2 - 10.1534/genetics.110.123893
DO - 10.1534/genetics.110.123893
M3 - Journal article
C2 - 21288872
VL - 187
SP - 1067
EP - 1075
JO - Genetics
JF - Genetics
SN - 1943-2631
IS - 4
ER -
ID: 38419000