Role for ribosome-associated quality control in sampling proteins for MHC class I-mediated antigen presentation
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Role for ribosome-associated quality control in sampling proteins for MHC class I-mediated antigen presentation. / Trentini, Débora Broch; Pecoraro, Matteo; Tiwary, Shivani; Cox, Jürgen; Mann, Matthias; Hipp, Mark S; Hartl, F Ulrich.
I: Proceedings of the National Academy of Sciences of the United States of America, Bind 117, Nr. 8, 25.02.2020, s. 4099-4108.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Role for ribosome-associated quality control in sampling proteins for MHC class I-mediated antigen presentation
AU - Trentini, Débora Broch
AU - Pecoraro, Matteo
AU - Tiwary, Shivani
AU - Cox, Jürgen
AU - Mann, Matthias
AU - Hipp, Mark S
AU - Hartl, F Ulrich
N1 - Copyright © 2020 the Author(s). Published by PNAS.
PY - 2020/2/25
Y1 - 2020/2/25
N2 - Mammalian cells present a fingerprint of their proteome to the adaptive immune system through the display of endogenous peptides on MHC-I complexes. MHC-I-bound peptides originate from protein degradation by the proteasome, suggesting that stably folded, long-lived proteins could evade monitoring. Here, we investigate the role in antigen presentation of the ribosome-associated quality control (RQC) pathway for the degradation of nascent polypeptides that are encoded by defective messenger RNAs and undergo stalling at the ribosome during translation. We find that degradation of model proteins by RQC results in efficient MHC-I presentation, independent of their intrinsic folding properties. Quantitative profiling of MHC-I peptides in wild-type and RQC-deficient cells by mass spectrometry showed that RQC substantially contributes to the composition of the immunopeptidome. Our results also identify endogenous substrates of the RQC pathway in human cells and provide insight into common principles causing ribosome stalling under physiological conditions.
AB - Mammalian cells present a fingerprint of their proteome to the adaptive immune system through the display of endogenous peptides on MHC-I complexes. MHC-I-bound peptides originate from protein degradation by the proteasome, suggesting that stably folded, long-lived proteins could evade monitoring. Here, we investigate the role in antigen presentation of the ribosome-associated quality control (RQC) pathway for the degradation of nascent polypeptides that are encoded by defective messenger RNAs and undergo stalling at the ribosome during translation. We find that degradation of model proteins by RQC results in efficient MHC-I presentation, independent of their intrinsic folding properties. Quantitative profiling of MHC-I peptides in wild-type and RQC-deficient cells by mass spectrometry showed that RQC substantially contributes to the composition of the immunopeptidome. Our results also identify endogenous substrates of the RQC pathway in human cells and provide insight into common principles causing ribosome stalling under physiological conditions.
U2 - 10.1073/pnas.1914401117
DO - 10.1073/pnas.1914401117
M3 - Journal article
C2 - 32047030
VL - 117
SP - 4099
EP - 4108
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 8
ER -
ID: 239206641