RiboMeth-seq: Profiling of 2'-O-Me in RNA
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
RiboMeth-seq is a sequencing-based method for mapping and quantitation of one of the most abundant RNA modifications, ribose methylation. It is based on a simple chemical principle, namely the several orders of magnitude difference in nucleophilicity of a 2'-OH and a 2'-O-Me. Thus, the method combines alkaline fragmentation and a specialized library construction protocol based on 5'-OH and 2',3' cyclic phosphate ends to prepare RNA for sequencing. The read-ends of library fragments are used for mapping with nucleotide resolution and calculation of the fraction of molecules methylated at the 2'-O-Me sites.
Originalsprog | Engelsk |
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Titel | RNA Methylation |
Antal sider | 21 |
Vol/bind | 1562 |
Forlag | Springer |
Publikationsdato | 2017 |
Sider | 189-209 |
ISBN (Trykt) | 978-1-4939-6805-3 |
ISBN (Elektronisk) | 978-1-4939-6807-7 |
DOI | |
Status | Udgivet - 2017 |
Navn | Methods in Molecular Biology |
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Vol/bind | 1562 |
ISSN | 1064-3745 |
ID: 180367464