RiboMeth-seq: Profiling of 2'-O-Me in RNA
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RiboMeth-seq : Profiling of 2'-O-Me in RNA. / Krogh, Nicolai; Birkedal, Ulf; Nielsen, Henrik.
RNA Methylation. Bind 1562 Springer, 2017. s. 189-209 (Methods in Molecular Biology, Bind 1562).Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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TY - CHAP
T1 - RiboMeth-seq
T2 - Profiling of 2'-O-Me in RNA
AU - Krogh, Nicolai
AU - Birkedal, Ulf
AU - Nielsen, Henrik
PY - 2017
Y1 - 2017
N2 - RiboMeth-seq is a sequencing-based method for mapping and quantitation of one of the most abundant RNA modifications, ribose methylation. It is based on a simple chemical principle, namely the several orders of magnitude difference in nucleophilicity of a 2'-OH and a 2'-O-Me. Thus, the method combines alkaline fragmentation and a specialized library construction protocol based on 5'-OH and 2',3' cyclic phosphate ends to prepare RNA for sequencing. The read-ends of library fragments are used for mapping with nucleotide resolution and calculation of the fraction of molecules methylated at the 2'-O-Me sites.
AB - RiboMeth-seq is a sequencing-based method for mapping and quantitation of one of the most abundant RNA modifications, ribose methylation. It is based on a simple chemical principle, namely the several orders of magnitude difference in nucleophilicity of a 2'-OH and a 2'-O-Me. Thus, the method combines alkaline fragmentation and a specialized library construction protocol based on 5'-OH and 2',3' cyclic phosphate ends to prepare RNA for sequencing. The read-ends of library fragments are used for mapping with nucleotide resolution and calculation of the fraction of molecules methylated at the 2'-O-Me sites.
U2 - 10.1007/978-1-4939-6807-7_13
DO - 10.1007/978-1-4939-6807-7_13
M3 - Book chapter
C2 - 28349462
SN - 978-1-4939-6805-3
VL - 1562
T3 - Methods in Molecular Biology
SP - 189
EP - 209
BT - RNA Methylation
PB - Springer
ER -
ID: 180367464