Rheumatoid factor and its interference with cytokine measurements: problems and solutions
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Rheumatoid factor and its interference with cytokine measurements : problems and solutions. / Bartels, Else Marie; Falbe Wätjen, Inger; Littrup Andersen, Eva; Danneskiold-Samsøe, Bente; Bliddal, Henning; Ribel-Madsen, Søren.
I: Arthritis Today, Bind 2011, 2011, s. 741071.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Rheumatoid factor and its interference with cytokine measurements
T2 - problems and solutions
AU - Bartels, Else Marie
AU - Falbe Wätjen, Inger
AU - Littrup Andersen, Eva
AU - Danneskiold-Samsøe, Bente
AU - Bliddal, Henning
AU - Ribel-Madsen, Søren
PY - 2011
Y1 - 2011
N2 - Use of cytokines as biomarkers for disease is getting more widespread. Cytokines are conveniently determined by immunoassay, but interference from present antibodies is known to cause problems. In rheumatoid arthritis (RA), interference of rheumatoid factor (RF) may be problematic. RF covers a group of autoantibodies from immunoglobulin subclasses and is present in 65-80% of RA patients. Partly removal of RF is possible by precipitation. This study aims at determining the effects of presence of RF in blood and synovial fluid on cytokine measurements in samples from RA patients and finding possible solutions for recognized problems. IL-1ß, IL-4, IL-6, and IL-8 were determined with multiplex immunoassays (MIA) in samples from RA patients prior to and after polyethylene glycol (PEG 6000) precipitation. Presence of RF does interfere with MIA. PEG 6000 precipitation abolishes this RF interference. We recommend PEG precipitation for all immunoassay measurements of plasma samples from RA patients.
AB - Use of cytokines as biomarkers for disease is getting more widespread. Cytokines are conveniently determined by immunoassay, but interference from present antibodies is known to cause problems. In rheumatoid arthritis (RA), interference of rheumatoid factor (RF) may be problematic. RF covers a group of autoantibodies from immunoglobulin subclasses and is present in 65-80% of RA patients. Partly removal of RF is possible by precipitation. This study aims at determining the effects of presence of RF in blood and synovial fluid on cytokine measurements in samples from RA patients and finding possible solutions for recognized problems. IL-1ß, IL-4, IL-6, and IL-8 were determined with multiplex immunoassays (MIA) in samples from RA patients prior to and after polyethylene glycol (PEG 6000) precipitation. Presence of RF does interfere with MIA. PEG 6000 precipitation abolishes this RF interference. We recommend PEG precipitation for all immunoassay measurements of plasma samples from RA patients.
U2 - http://dx.doi.org/10.1155/2011/741071
DO - http://dx.doi.org/10.1155/2011/741071
M3 - Journal article
VL - 2011
SP - 741071
JO - Arthritis Today
JF - Arthritis Today
SN - 0969-7039
ER -
ID: 40145863