TY - JOUR

T1 - Retinol-Binding Protein 4 in Young Men With Low Versus Normal Birth Weight

T2 - Physiological Response to Short-Term Overfeeding

AU - Ribel-Madsen, Rasmus

AU - Brøns, Charlotte

AU - Friedrichsen, Martin

AU - Poulsen, Pernille

AU - Vaag, Allan

PY - 2011/6/1

Y1 - 2011/6/1

N2 - Retinol-binding protein 4 (RBP4) is a plasma protein which is elevated in obesity and type 2 diabetes. We aimed to investigate whether RBP4 represents a mechanism underlying the associations between low birth weight (LBW), high-fat diet, and insulin resistance. Forty-six young, lean men with low (n = 20) or normal (n = 26) birth weight underwent a 5-day high-fat high-calorie (HFHC) dietary intervention. In vivo glucose metabolism was assessed by euglycemic-hyperinsulinemic clamp, glucose tracer and intravenous glucose tolerance test techniques. Body composition was measured by a dual-energy x-ray absorptiometry scan, and plasma RBP4 by an enzyme-linked immunosorbent assay. RBP4 was not associated with birth weight, but with BMI (ß = 0.9 µg/ml (0.08; 1.8) (95% confidence interval), P = 0.03) and plasma levels of low-density lipoprotein cholesterol (ß = 5.3 µg/ml (1.9; 8.7), P = 0.03) and triglycerides (ß = 15.4 µg/ml (9.5; 21.3), P <0.001). Under baseline diet conditions, RBP4 was associated with decreased disposition index (D(i)) (ß = -2.4% (-4.5%; -0.2%), P = 0.04) and increased basal hepatic glucose production rate (HGP) (ß = 0.02 mg kg(-1) min(-1) (0.002; 0.04), P = 0.03), but not associated with peripheral glucose disposal rate or hepatic insulin resistance index. RBP4 levels were not influenced by overfeeding or related to peripheral and hepatic insulin resistance provoked by the dietary intervention. In conclusion, plasma RBP4 in young men associates with components of the metabolic syndrome, but is not determined by birth weight and seems not to be involved in short-term high-fat diet-induced insulin resistance.

AB - Retinol-binding protein 4 (RBP4) is a plasma protein which is elevated in obesity and type 2 diabetes. We aimed to investigate whether RBP4 represents a mechanism underlying the associations between low birth weight (LBW), high-fat diet, and insulin resistance. Forty-six young, lean men with low (n = 20) or normal (n = 26) birth weight underwent a 5-day high-fat high-calorie (HFHC) dietary intervention. In vivo glucose metabolism was assessed by euglycemic-hyperinsulinemic clamp, glucose tracer and intravenous glucose tolerance test techniques. Body composition was measured by a dual-energy x-ray absorptiometry scan, and plasma RBP4 by an enzyme-linked immunosorbent assay. RBP4 was not associated with birth weight, but with BMI (ß = 0.9 µg/ml (0.08; 1.8) (95% confidence interval), P = 0.03) and plasma levels of low-density lipoprotein cholesterol (ß = 5.3 µg/ml (1.9; 8.7), P = 0.03) and triglycerides (ß = 15.4 µg/ml (9.5; 21.3), P <0.001). Under baseline diet conditions, RBP4 was associated with decreased disposition index (D(i)) (ß = -2.4% (-4.5%; -0.2%), P = 0.04) and increased basal hepatic glucose production rate (HGP) (ß = 0.02 mg kg(-1) min(-1) (0.002; 0.04), P = 0.03), but not associated with peripheral glucose disposal rate or hepatic insulin resistance index. RBP4 levels were not influenced by overfeeding or related to peripheral and hepatic insulin resistance provoked by the dietary intervention. In conclusion, plasma RBP4 in young men associates with components of the metabolic syndrome, but is not determined by birth weight and seems not to be involved in short-term high-fat diet-induced insulin resistance.

KW - Adult

KW - Birth Weight

KW - Body Mass Index

KW - Cholesterol, LDL

KW - Cross-Over Studies

KW - Dietary Fats

KW - Glucose

KW - Humans

KW - Infant Nutrition Disorders

KW - Infant, Low Birth Weight

KW - Infant, Newborn

KW - Insulin Resistance

KW - Liver

KW - Male

KW - Metabolic Syndrome X

KW - Overnutrition

KW - Retinol-Binding Proteins, Plasma

KW - Thinness

KW - Triglycerides

KW - Vitamin A

KW - Young Adult

U2 - 10.1038/oby.2010.311

DO - 10.1038/oby.2010.311

M3 - Journal article

C2 - 21164503

VL - 19

SP - 1304

EP - 1306

JO - Obesity

JF - Obesity

SN - 1930-7381

IS - 6

ER -