Reconstructing human pancreatic differentiation by mapping specific cell populations during development
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Reconstructing human pancreatic differentiation by mapping specific cell populations during development. / Ramond, Cyrille; Glaser, Nicolas; Berthault, Claire; Ameri, Jacqueline; Kirkegaard, Jeannette Schlichting; Hansson, Mattias; Honoré, Christian; Semb, Tor Henrik; Scharfmann, Raphaël.
I: eLife, Bind 6, e27564, 2017.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Reconstructing human pancreatic differentiation by mapping specific cell populations during development
AU - Ramond, Cyrille
AU - Glaser, Nicolas
AU - Berthault, Claire
AU - Ameri, Jacqueline
AU - Kirkegaard, Jeannette Schlichting
AU - Hansson, Mattias
AU - Honoré, Christian
AU - Semb, Tor Henrik
AU - Scharfmann, Raphaël
PY - 2017
Y1 - 2017
N2 - Information remains scarce on human development compared to animal models. Here, we reconstructed human fetal pancreatic differentiation using cell surface markers. We demonstrate that at 7weeks of development, the glycoprotein 2 (GP2) marks a multipotent cell population that will differentiate into the acinar, ductal or endocrine lineages. Development towards the acinar lineage is paralleled by an increase in GP2 expression. Conversely, a subset of the GP2+population undergoes endocrine differentiation by down-regulating GP2 and CD142 and turning on NEUROG3, a marker of endocrine differentiation. Endocrine maturation progresses by up-regulating SUSD2 and lowering ECAD levels. Finally, in vitro differentiation of pancreatic endocrine cells derived from human pluripotent stem cells mimics key in vivo events. Our work paves the way to extend our understanding of the origin of mature human pancreatic cell types and how such lineage decisions are regulated.
AB - Information remains scarce on human development compared to animal models. Here, we reconstructed human fetal pancreatic differentiation using cell surface markers. We demonstrate that at 7weeks of development, the glycoprotein 2 (GP2) marks a multipotent cell population that will differentiate into the acinar, ductal or endocrine lineages. Development towards the acinar lineage is paralleled by an increase in GP2 expression. Conversely, a subset of the GP2+population undergoes endocrine differentiation by down-regulating GP2 and CD142 and turning on NEUROG3, a marker of endocrine differentiation. Endocrine maturation progresses by up-regulating SUSD2 and lowering ECAD levels. Finally, in vitro differentiation of pancreatic endocrine cells derived from human pluripotent stem cells mimics key in vivo events. Our work paves the way to extend our understanding of the origin of mature human pancreatic cell types and how such lineage decisions are regulated.
U2 - 10.7554/eLife.27564
DO - 10.7554/eLife.27564
M3 - Journal article
C2 - 28731406
AN - SCOPUS:85026906029
VL - 6
JO - eLife
JF - eLife
SN - 2050-084X
M1 - e27564
ER -
ID: 196141519