Reconstitution of ATP-dependent lipid transporters: gaining insight into molecular characteristics, regulation, and mechanisms
Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt
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Reconstitution of ATP-dependent lipid transporters : gaining insight into molecular characteristics, regulation, and mechanisms. / Herrera, Sara Abad; Pomorski, Thomas Günther.
I: Bioscience Reports, Bind 43, Nr. 8, 2023.Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt
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TY - JOUR
T1 - Reconstitution of ATP-dependent lipid transporters
T2 - gaining insight into molecular characteristics, regulation, and mechanisms
AU - Herrera, Sara Abad
AU - Pomorski, Thomas Günther
N1 - Publisher Copyright: © 2023 The Author(s).
PY - 2023
Y1 - 2023
N2 - Lipid transporters play a crucial role in supporting essential cellular processes such as organelle assembly, vesicular trafficking, and lipid homeostasis by driving lipid transport across membranes. Cryo-electron microscopy has recently resolved the structures of several ATP-dependent lipid transporters, but functional characterization remains a major challenge. Although studies of detergent-purified proteins have advanced our understanding of these transporters, in vitro evidence for lipid transport is still limited to a few ATP-dependent lipid transporters. Reconstitution into model membranes, such as liposomes, is a suitable approach to study lipid transporters in vitro and to investigate their key molecular features. In this review, we discuss the current approaches for reconstituting ATP-driven lipid transporters into large liposomes and common techniques used to study lipid transport in proteoliposomes. We also highlight the existing knowledge on the regulatory mechanisms that modulate the activity of lipid transporters, and finally, we address the limitations of the current approaches and future perspectives in this field.
AB - Lipid transporters play a crucial role in supporting essential cellular processes such as organelle assembly, vesicular trafficking, and lipid homeostasis by driving lipid transport across membranes. Cryo-electron microscopy has recently resolved the structures of several ATP-dependent lipid transporters, but functional characterization remains a major challenge. Although studies of detergent-purified proteins have advanced our understanding of these transporters, in vitro evidence for lipid transport is still limited to a few ATP-dependent lipid transporters. Reconstitution into model membranes, such as liposomes, is a suitable approach to study lipid transporters in vitro and to investigate their key molecular features. In this review, we discuss the current approaches for reconstituting ATP-driven lipid transporters into large liposomes and common techniques used to study lipid transport in proteoliposomes. We also highlight the existing knowledge on the regulatory mechanisms that modulate the activity of lipid transporters, and finally, we address the limitations of the current approaches and future perspectives in this field.
KW - ABC transporter
KW - Flipppase
KW - Liposomes
KW - P-type ATPase
KW - Reconstitution
U2 - 10.1042/BSR20221268
DO - 10.1042/BSR20221268
M3 - Review
C2 - 37417269
AN - SCOPUS:85167480776
VL - 43
JO - Bioscience Reports
JF - Bioscience Reports
SN - 0144-8463
IS - 8
ER -
ID: 363061409