Reciprocal GLUT-1 and GLUT-4 expression and glucose transport in denervated muscles
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Reciprocal GLUT-1 and GLUT-4 expression and glucose transport in denervated muscles. / Handberg, A.; Megeney, L. A.; McCullagh, K. J.A.; Kayser, L.; Han, X. X.; Bonen, A.
I: American Journal of Physiology - Endocrinology and Metabolism, Bind 271, Nr. 1, 1996, s. E50-E57.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Reciprocal GLUT-1 and GLUT-4 expression and glucose transport in denervated muscles
AU - Handberg, A.
AU - Megeney, L. A.
AU - McCullagh, K. J.A.
AU - Kayser, L.
AU - Han, X. X.
AU - Bonen, A.
PY - 1996
Y1 - 1996
N2 - We investigated in 3-day-denervated muscles 1) the expression of GLUT-1 in perineurial sheaths (PNS) and muscle, 2) the muscle fiber-specific changes in GLUT-1 and GLUT-4, and 3) changes in basal and insulin-stimulated 3-O- methylglucose transport. GLUT-1 was increased in both the PNS (P < 0.05) and in the muscle membranes (P < 0.05). GLUT-1 and GLUT-4 concentrations were changed reciprocally, in a fiber-dependent fashion [GLUT-1: red gastrocnemius (RG), + 31%; white gastrocnemius (WG), +10%; GLUT-4: RG, -53%; WG, -16%]. Basal glucose transport was increased (P < 0.05), and this increase was correlated with the oxidative nature of the muscles (r = 0.97). Insulin- stimulated glucose transport was decreased in denervated muscles (P < 0.05). This was also related to the oxidative nature of the muscles (r = -0.88). The increase in basal glucose transport was correlated with the loss of insulin- stimulated transport (r = 0.95). Thus the increase in GLUT-1 compensates for the loss of GLUT-4, resulting in a 56% regain of the reduced insulin- stimulated glucose transport.
AB - We investigated in 3-day-denervated muscles 1) the expression of GLUT-1 in perineurial sheaths (PNS) and muscle, 2) the muscle fiber-specific changes in GLUT-1 and GLUT-4, and 3) changes in basal and insulin-stimulated 3-O- methylglucose transport. GLUT-1 was increased in both the PNS (P < 0.05) and in the muscle membranes (P < 0.05). GLUT-1 and GLUT-4 concentrations were changed reciprocally, in a fiber-dependent fashion [GLUT-1: red gastrocnemius (RG), + 31%; white gastrocnemius (WG), +10%; GLUT-4: RG, -53%; WG, -16%]. Basal glucose transport was increased (P < 0.05), and this increase was correlated with the oxidative nature of the muscles (r = 0.97). Insulin- stimulated glucose transport was decreased in denervated muscles (P < 0.05). This was also related to the oxidative nature of the muscles (r = -0.88). The increase in basal glucose transport was correlated with the loss of insulin- stimulated transport (r = 0.95). Thus the increase in GLUT-1 compensates for the loss of GLUT-4, resulting in a 56% regain of the reduced insulin- stimulated glucose transport.
KW - 3-O-methylglucose transport
KW - hindlimb perfusion
KW - immunofluorescence
KW - insulin
KW - muscle fiber composition
KW - perineurial sheaths
KW - plasma membranes
U2 - 10.1152/ajpendo.1996.271.1.e50
DO - 10.1152/ajpendo.1996.271.1.e50
M3 - Journal article
C2 - 8760081
AN - SCOPUS:0029788424
VL - 271
SP - E50-E57
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
SN - 0193-1849
IS - 1
ER -
ID: 335353289