Rat insulinoma cells express both a 115-kDa growth hormone receptor and a 95-kDa prolactin receptor structurally related to the hepatic receptors
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Rat insulinoma cells express both a 115-kDa growth hormone receptor and a 95-kDa prolactin receptor structurally related to the hepatic receptors. / Møldrup, Annette; Billestrup, N; Nielsen, Jens Høiriis.
I: The Journal of Biological Chemistry, Bind 265, Nr. 15, 25.05.1990, s. 8686-90.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Rat insulinoma cells express both a 115-kDa growth hormone receptor and a 95-kDa prolactin receptor structurally related to the hepatic receptors
AU - Møldrup, Annette
AU - Billestrup, N
AU - Nielsen, Jens Høiriis
PY - 1990/5/25
Y1 - 1990/5/25
N2 - Insulin-producing rat islet RIN-5AH tumor cells express multiple binding sites for human growth hormone (hGH). The effect of rat growth hormone (rGH), rat prolactin (rPRL), and human placental lactogen (hPL) on the binding of 125I-labeled hGH (125I-hGH) to RIN-5AH cells revealed the presence of both lactogen and somatogen receptor populations. Covalent cross-linking of 125I-hGH, 125I-rGH, and 125I-rPRL to the RIN cells identified a 115-kDa somatogen receptor protein that binds hGH and rGH but not rPRL and hPL, and a 95-kDa lactogen receptor protein that binds hGH, rPRL, and hPL but not rGH. Antiserum directed against the 37.5- and 40.7-kDa GH-binding proteins of mouse hepatic tissue specifically recognized the 115-kDa protein cross-linked with 125I-hGH, whereas a monoclonal antibody raised against the hepatic 42-kDa rPRL receptor recognized the 95-kDa protein cross-linked with 125I-hGH. Northern blot analysis of RNA from RIN-5AH cells using an rGH receptor-specific cDNA probe showed three transcripts of 5.8, 1.8, and 1.5 kilobases, respectively. Analysis with an rPRL receptor-specific cDNA probe revealed the presence of a predominant 3.5-kilobase transcript. We conclude that the insulin-producing cells express large forms of both the GH and PRL receptors which are structurally related to those of hepatic tissue.
AB - Insulin-producing rat islet RIN-5AH tumor cells express multiple binding sites for human growth hormone (hGH). The effect of rat growth hormone (rGH), rat prolactin (rPRL), and human placental lactogen (hPL) on the binding of 125I-labeled hGH (125I-hGH) to RIN-5AH cells revealed the presence of both lactogen and somatogen receptor populations. Covalent cross-linking of 125I-hGH, 125I-rGH, and 125I-rPRL to the RIN cells identified a 115-kDa somatogen receptor protein that binds hGH and rGH but not rPRL and hPL, and a 95-kDa lactogen receptor protein that binds hGH, rPRL, and hPL but not rGH. Antiserum directed against the 37.5- and 40.7-kDa GH-binding proteins of mouse hepatic tissue specifically recognized the 115-kDa protein cross-linked with 125I-hGH, whereas a monoclonal antibody raised against the hepatic 42-kDa rPRL receptor recognized the 95-kDa protein cross-linked with 125I-hGH. Northern blot analysis of RNA from RIN-5AH cells using an rGH receptor-specific cDNA probe showed three transcripts of 5.8, 1.8, and 1.5 kilobases, respectively. Analysis with an rPRL receptor-specific cDNA probe revealed the presence of a predominant 3.5-kilobase transcript. We conclude that the insulin-producing cells express large forms of both the GH and PRL receptors which are structurally related to those of hepatic tissue.
KW - Adenoma, Islet Cell
KW - Animals
KW - Blotting, Northern
KW - Cell Line
KW - Cross-Linking Reagents
KW - Growth Hormone
KW - Insulinoma
KW - Kinetics
KW - Liver
KW - Molecular Weight
KW - Pancreatic Neoplasms
KW - RNA
KW - RNA, Neoplasm
KW - Rats
KW - Receptors, Prolactin
KW - Receptors, Somatotropin
M3 - Journal article
C2 - 1692834
VL - 265
SP - 8686
EP - 8690
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 15
ER -
ID: 47974053