Rad52 and Rad59 exhibit both overlapping and distinct functions

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Rad52 and Rad59 exhibit both overlapping and distinct functions. / Feng, Qi; Düring, Louis; de Mayolo, Adriana Antúnez; Buchhorn, Gaëlle Lettier; Lisby, Michael; Erdeniz, Naz; Mortensen, Uffe H; Rothstein, Rodney.

I: DNA Repair, Bind 6, Nr. 1, 2007, s. 27-37.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Feng, Q, Düring, L, de Mayolo, AA, Buchhorn, GL, Lisby, M, Erdeniz, N, Mortensen, UH & Rothstein, R 2007, 'Rad52 and Rad59 exhibit both overlapping and distinct functions', DNA Repair, bind 6, nr. 1, s. 27-37. https://doi.org/10.1016/j.dnarep.2006.08.007

APA

Feng, Q., Düring, L., de Mayolo, A. A., Buchhorn, G. L., Lisby, M., Erdeniz, N., Mortensen, U. H., & Rothstein, R. (2007). Rad52 and Rad59 exhibit both overlapping and distinct functions. DNA Repair, 6(1), 27-37. https://doi.org/10.1016/j.dnarep.2006.08.007

Vancouver

Feng Q, Düring L, de Mayolo AA, Buchhorn GL, Lisby M, Erdeniz N o.a. Rad52 and Rad59 exhibit both overlapping and distinct functions. DNA Repair. 2007;6(1):27-37. https://doi.org/10.1016/j.dnarep.2006.08.007

Author

Feng, Qi ; Düring, Louis ; de Mayolo, Adriana Antúnez ; Buchhorn, Gaëlle Lettier ; Lisby, Michael ; Erdeniz, Naz ; Mortensen, Uffe H ; Rothstein, Rodney. / Rad52 and Rad59 exhibit both overlapping and distinct functions. I: DNA Repair. 2007 ; Bind 6, Nr. 1. s. 27-37.

Bibtex

@article{a4026ce0eba011ddbf70000ea68e967b,

title = "Rad52 and Rad59 exhibit both overlapping and distinct functions",

abstract = "Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.",

author = "Qi Feng and Louis D{\"u}ring and {de Mayolo}, {Adriana Ant{\'u}nez} and Buchhorn, {Ga{\"e}lle Lettier} and Michael Lisby and Naz Erdeniz and Mortensen, {Uffe H} and Rodney Rothstein",

year = "2007",

doi = "10.1016/j.dnarep.2006.08.007",

language = "English",

volume = "6",

pages = "27--37",

journal = "DNA Repair",

issn = "1568-7864",

publisher = "Elsevier",

number = "1",

}

RIS

TY - JOUR

T1 - Rad52 and Rad59 exhibit both overlapping and distinct functions

AU - Feng, Qi

AU - Düring, Louis

AU - de Mayolo, Adriana Antúnez

AU - Buchhorn, Gaëlle Lettier

AU - Lisby, Michael

AU - Erdeniz, Naz

AU - Mortensen, Uffe H

AU - Rothstein, Rodney

PY - 2007

Y1 - 2007

N2 - Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.

AB - Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.

U2 - 10.1016/j.dnarep.2006.08.007

DO - 10.1016/j.dnarep.2006.08.007

M3 - Journal article

VL - 6

SP - 27

EP - 37

JO - DNA Repair

JF - DNA Repair

SN - 1568-7864

IS - 1

ER -

ID: 9937509