Rad52 and Rad59 exhibit both overlapping and distinct functions
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Rad52 and Rad59 exhibit both overlapping and distinct functions. / Feng, Qi; Düring, Louis; de Mayolo, Adriana Antúnez; Buchhorn, Gaëlle Lettier; Lisby, Michael; Erdeniz, Naz; Mortensen, Uffe H; Rothstein, Rodney.
I: DNA Repair, Bind 6, Nr. 1, 2007, s. 27-37.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Rad52 and Rad59 exhibit both overlapping and distinct functions
AU - Feng, Qi
AU - Düring, Louis
AU - de Mayolo, Adriana Antúnez
AU - Buchhorn, Gaëlle Lettier
AU - Lisby, Michael
AU - Erdeniz, Naz
AU - Mortensen, Uffe H
AU - Rothstein, Rodney
PY - 2007
Y1 - 2007
N2 - Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.
AB - Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.
U2 - 10.1016/j.dnarep.2006.08.007
DO - 10.1016/j.dnarep.2006.08.007
M3 - Journal article
VL - 6
SP - 27
EP - 37
JO - DNA Repair
JF - DNA Repair
SN - 1568-7864
IS - 1
ER -
ID: 9937509