Quantity and Quality of Basophil RNA Depend on the RNA Extraction Technique
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
Basophils have been suggested to express low quantities of RNA, challenging the study of gene expression within these cells. However, the purification technique employed might have an impact on the quantity and quality of RNA purified from basophils. This chapter describes a method which gives an optimal RNA output using a TRIzol-based method in contrast to a commercial kit.
Originalsprog | Engelsk |
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Titel | Basophils and Mast Cells : Methods and Protocols |
Redaktører | Bernhard F. Gibbs, Franco H. Falcone |
Vol/bind | 2163 |
Forlag | Humana Press |
Publikationsdato | 2020 |
Sider | 241-245 |
ISBN (Trykt) | 978-1-0716-0695-7 |
ISBN (Elektronisk) | 978-1-0716-0696-4 |
DOI | |
Status | Udgivet - 2020 |
Navn | Methods in Molecular Biology |
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Vol/bind | 2163 |
ISSN | 1064-3745 |
ID: 251307977