Quality assessment of a placental perfusion protocol

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Standard

Quality assessment of a placental perfusion protocol. / Mathiesen, Line; Mose, Tina; Mørck, Thit Juul; Nielsen, Jeanette Kolstrup Søgaard; Nielsen, Leif Kofoed; Maroun, Lisa Leth; Dziegiel, Morten Hanefeld; Larsen, Lise Grupe; Knudsen, Lisbeth E.

I: Reproductive Toxicology, Bind 30, Nr. 1, 08.2010, s. 138-46.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Mathiesen, L, Mose, T, Mørck, TJ, Nielsen, JKS, Nielsen, LK, Maroun, LL, Dziegiel, MH, Larsen, LG & Knudsen, LE 2010, 'Quality assessment of a placental perfusion protocol', Reproductive Toxicology, bind 30, nr. 1, s. 138-46. https://doi.org/10.1016/j.reprotox.2010.01.006

APA

Mathiesen, L., Mose, T., Mørck, T. J., Nielsen, J. K. S., Nielsen, L. K., Maroun, L. L., Dziegiel, M. H., Larsen, L. G., & Knudsen, L. E. (2010). Quality assessment of a placental perfusion protocol. Reproductive Toxicology, 30(1), 138-46. https://doi.org/10.1016/j.reprotox.2010.01.006

Vancouver

Mathiesen L, Mose T, Mørck TJ, Nielsen JKS, Nielsen LK, Maroun LL o.a. Quality assessment of a placental perfusion protocol. Reproductive Toxicology. 2010 aug.;30(1):138-46. https://doi.org/10.1016/j.reprotox.2010.01.006

Author

Mathiesen, Line ; Mose, Tina ; Mørck, Thit Juul ; Nielsen, Jeanette Kolstrup Søgaard ; Nielsen, Leif Kofoed ; Maroun, Lisa Leth ; Dziegiel, Morten Hanefeld ; Larsen, Lise Grupe ; Knudsen, Lisbeth E. / Quality assessment of a placental perfusion protocol. I: Reproductive Toxicology. 2010 ; Bind 30, Nr. 1. s. 138-46.

Bibtex

@article{4d810e50835011df928f000ea68e967b,

title = "Quality assessment of a placental perfusion protocol",

abstract = "Validation of in vitro test systems using the modular approach with steps addressing reliability and relevance is an important aim when developing in vitro tests in e.g. reproductive toxicology. The ex vivo human placental perfusion system may be used for such validation, here presenting the placental perfusion model in Copenhagen including control substances. The positive control substance antipyrine shows no difference in transport regardless of perfusion media used or of terms of delivery (n=59, p<0.05). Negative control studies with FITC marked dextran correspond with leakage criteria (<3 ml h(-1) from the fetal reservoir) when adding 2 (n=7) and 20mg (n=9) FITC-dextran/100 ml fetal perfusion media. Success rate of the Copenhagen placental perfusions is provided in this study, including considerations and quality control parameters. Three checkpoints suggested to determine success rate revealed that 15% of the cannulated placentae received in one year (n=202) were successfully perfused.",

keywords = "Animal Testing Alternatives, Antipyrine, Equipment Design, Female, Humans, In Vitro Techniques, Maternal-Fetal Exchange, Perfusion, Placenta, Pregnancy, Quality Control, Reproduction, Toxicity Tests",

author = "Line Mathiesen and Tina Mose and M{\o}rck, {Thit Juul} and Nielsen, {Jeanette Kolstrup S{\o}gaard} and Nielsen, {Leif Kofoed} and Maroun, {Lisa Leth} and Dziegiel, {Morten Hanefeld} and Larsen, {Lise Grupe} and Knudsen, {Lisbeth E.}",

year = "2010",

month = aug,

doi = "10.1016/j.reprotox.2010.01.006",

language = "English",

volume = "30",

pages = "138--46",

journal = "Reproductive Toxicology",

issn = "0890-6238",

publisher = "Elsevier",

number = "1",

}

RIS

TY - JOUR

T1 - Quality assessment of a placental perfusion protocol

AU - Mathiesen, Line

AU - Mose, Tina

AU - Mørck, Thit Juul

AU - Nielsen, Jeanette Kolstrup Søgaard

AU - Nielsen, Leif Kofoed

AU - Maroun, Lisa Leth

AU - Dziegiel, Morten Hanefeld

AU - Larsen, Lise Grupe

AU - Knudsen, Lisbeth E.

PY - 2010/8

Y1 - 2010/8

N2 - Validation of in vitro test systems using the modular approach with steps addressing reliability and relevance is an important aim when developing in vitro tests in e.g. reproductive toxicology. The ex vivo human placental perfusion system may be used for such validation, here presenting the placental perfusion model in Copenhagen including control substances. The positive control substance antipyrine shows no difference in transport regardless of perfusion media used or of terms of delivery (n=59, p<0.05). Negative control studies with FITC marked dextran correspond with leakage criteria (<3 ml h(-1) from the fetal reservoir) when adding 2 (n=7) and 20mg (n=9) FITC-dextran/100 ml fetal perfusion media. Success rate of the Copenhagen placental perfusions is provided in this study, including considerations and quality control parameters. Three checkpoints suggested to determine success rate revealed that 15% of the cannulated placentae received in one year (n=202) were successfully perfused.

AB - Validation of in vitro test systems using the modular approach with steps addressing reliability and relevance is an important aim when developing in vitro tests in e.g. reproductive toxicology. The ex vivo human placental perfusion system may be used for such validation, here presenting the placental perfusion model in Copenhagen including control substances. The positive control substance antipyrine shows no difference in transport regardless of perfusion media used or of terms of delivery (n=59, p<0.05). Negative control studies with FITC marked dextran correspond with leakage criteria (<3 ml h(-1) from the fetal reservoir) when adding 2 (n=7) and 20mg (n=9) FITC-dextran/100 ml fetal perfusion media. Success rate of the Copenhagen placental perfusions is provided in this study, including considerations and quality control parameters. Three checkpoints suggested to determine success rate revealed that 15% of the cannulated placentae received in one year (n=202) were successfully perfused.

KW - Animal Testing Alternatives

KW - Antipyrine

KW - Equipment Design

KW - Female

KW - Humans

KW - In Vitro Techniques

KW - Maternal-Fetal Exchange

KW - Perfusion

KW - Placenta

KW - Pregnancy

KW - Quality Control

KW - Reproduction

KW - Toxicity Tests

U2 - 10.1016/j.reprotox.2010.01.006

DO - 10.1016/j.reprotox.2010.01.006

M3 - Journal article

C2 - 20096346

VL - 30

SP - 138

EP - 146

JO - Reproductive Toxicology

JF - Reproductive Toxicology

SN - 0890-6238

IS - 1

ER -

ID: 20569607