Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts

Publikation: Bidrag til tidsskrift › Konferenceartikel › Forskning › fagfællebedømt

Standard

Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts. / Maria Irlund Pedersen, Line; Stangegaard, Michael; Mogensen, Helle Smidt; Morling, Niels.

I: Forensic Science International: Genetics Supplement Series, Bind 3, Nr. 1, 2011, s. e347-e348.

Publikation: Bidrag til tidsskrift › Konferenceartikel › Forskning › fagfællebedømt

Harvard

Maria Irlund Pedersen, L, Stangegaard, M, Mogensen, HS & Morling, N 2011, 'Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts', Forensic Science International: Genetics Supplement Series, bind 3, nr. 1, s. e347-e348. https://doi.org/doi:10.1016/j.fsigss.2011.09.036

APA

Maria Irlund Pedersen, L., Stangegaard, M., Mogensen, H. S., & Morling, N. (2011). Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts. Forensic Science International: Genetics Supplement Series, 3(1), e347-e348. https://doi.org/doi:10.1016/j.fsigss.2011.09.036

Vancouver

Maria Irlund Pedersen L, Stangegaard M, Mogensen HS, Morling N. Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts. Forensic Science International: Genetics Supplement Series. 2011;3(1):e347-e348. https://doi.org/doi:10.1016/j.fsigss.2011.09.036

Author

Maria Irlund Pedersen, Line ; Stangegaard, Michael ; Mogensen, Helle Smidt ; Morling, Niels. / Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts. I: Forensic Science International: Genetics Supplement Series. 2011 ; Bind 3, Nr. 1. s. e347-e348.

Bibtex

@inproceedings{2ef472d5fedb49ab86c7d7fef9e7ea9a,
title = "Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts",
abstract = "Capillary electrophoresis of amplified DNA isolated from trace evidence samples occasionally results in inadequate STR-profiles due to artifacts caused by e.g. primers and dNTPs. Removal of artifacts by purification and subsequent concentration of the PCR products may increase the sensitivity and the quality of the DNA profiles without re-amplification of the sample. We have validated and implemented an automated method to purify and 2-fold concentrate PCR products resulting in allelic peaks with higher intensity (a median height across all loci from 130 to 404 RFU), fewer allelic dropouts and a reduced number of artifacts compared to both an increase in injection time and increase in the number of amplification cycles.",
author = "{Maria Irlund Pedersen}, Line and Michael Stangegaard and Mogensen, {Helle Smidt} and Niels Morling",
year = "2011",
doi = "doi:10.1016/j.fsigss.2011.09.036",
language = "English",
volume = "3",
pages = "e347--e348",
journal = "Forensic Science International: Genetics. Supplement Series",
issn = "1875-1768",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

RIS

TY - GEN

T1 - Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts

AU - Maria Irlund Pedersen, Line

AU - Stangegaard, Michael

AU - Mogensen, Helle Smidt

AU - Morling, Niels

PY - 2011

Y1 - 2011

N2 - Capillary electrophoresis of amplified DNA isolated from trace evidence samples occasionally results in inadequate STR-profiles due to artifacts caused by e.g. primers and dNTPs. Removal of artifacts by purification and subsequent concentration of the PCR products may increase the sensitivity and the quality of the DNA profiles without re-amplification of the sample. We have validated and implemented an automated method to purify and 2-fold concentrate PCR products resulting in allelic peaks with higher intensity (a median height across all loci from 130 to 404 RFU), fewer allelic dropouts and a reduced number of artifacts compared to both an increase in injection time and increase in the number of amplification cycles.

AB - Capillary electrophoresis of amplified DNA isolated from trace evidence samples occasionally results in inadequate STR-profiles due to artifacts caused by e.g. primers and dNTPs. Removal of artifacts by purification and subsequent concentration of the PCR products may increase the sensitivity and the quality of the DNA profiles without re-amplification of the sample. We have validated and implemented an automated method to purify and 2-fold concentrate PCR products resulting in allelic peaks with higher intensity (a median height across all loci from 130 to 404 RFU), fewer allelic dropouts and a reduced number of artifacts compared to both an increase in injection time and increase in the number of amplification cycles.

U2 - doi:10.1016/j.fsigss.2011.09.036

DO - doi:10.1016/j.fsigss.2011.09.036

M3 - Conference article

VL - 3

SP - e347-e348

JO - Forensic Science International: Genetics. Supplement Series

JF - Forensic Science International: Genetics. Supplement Series

SN - 1875-1768

IS - 1

ER -

ID: 38238667