Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts
Publikation: Bidrag til tidsskrift › Konferenceartikel › Forskning › fagfællebedømt
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Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts. / Maria Irlund Pedersen, Line; Stangegaard, Michael; Mogensen, Helle Smidt; Morling, Niels.
I: Forensic Science International: Genetics Supplement Series, Bind 3, Nr. 1, 2011, s. e347-e348.Publikation: Bidrag til tidsskrift › Konferenceartikel › Forskning › fagfællebedømt
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TY - GEN
T1 - Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts
AU - Maria Irlund Pedersen, Line
AU - Stangegaard, Michael
AU - Mogensen, Helle Smidt
AU - Morling, Niels
PY - 2011
Y1 - 2011
N2 - Capillary electrophoresis of amplified DNA isolated from trace evidence samples occasionally results in inadequate STR-profiles due to artifacts caused by e.g. primers and dNTPs. Removal of artifacts by purification and subsequent concentration of the PCR products may increase the sensitivity and the quality of the DNA profiles without re-amplification of the sample. We have validated and implemented an automated method to purify and 2-fold concentrate PCR products resulting in allelic peaks with higher intensity (a median height across all loci from 130 to 404 RFU), fewer allelic dropouts and a reduced number of artifacts compared to both an increase in injection time and increase in the number of amplification cycles.
AB - Capillary electrophoresis of amplified DNA isolated from trace evidence samples occasionally results in inadequate STR-profiles due to artifacts caused by e.g. primers and dNTPs. Removal of artifacts by purification and subsequent concentration of the PCR products may increase the sensitivity and the quality of the DNA profiles without re-amplification of the sample. We have validated and implemented an automated method to purify and 2-fold concentrate PCR products resulting in allelic peaks with higher intensity (a median height across all loci from 130 to 404 RFU), fewer allelic dropouts and a reduced number of artifacts compared to both an increase in injection time and increase in the number of amplification cycles.
U2 - doi:10.1016/j.fsigss.2011.09.036
DO - doi:10.1016/j.fsigss.2011.09.036
M3 - Conference article
VL - 3
SP - e347-e348
JO - Forensic Science International: Genetics. Supplement Series
JF - Forensic Science International: Genetics. Supplement Series
SN - 1875-1768
IS - 1
ER -
ID: 38238667