Protoporphyrin IX formation and photobleaching in different layers of normal human skin: methyl- and hexylaminolevulinate and different light sources

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Standard

Protoporphyrin IX formation and photobleaching in different layers of normal human skin : methyl- and hexylaminolevulinate and different light sources. / Togsverd-Bo, Katrine; Idorn, Luise W; Philipsen, Peter A; Wulf, Hans Christian; Hædersdal, Merete.

I: Experimental Dermatology Online, Bind 21, Nr. 10, 2012, s. 745-750.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Togsverd-Bo, K, Idorn, LW, Philipsen, PA, Wulf, HC & Hædersdal, M 2012, 'Protoporphyrin IX formation and photobleaching in different layers of normal human skin: methyl- and hexylaminolevulinate and different light sources', Experimental Dermatology Online, bind 21, nr. 10, s. 745-750. https://doi.org/10.1111/j.1600-0625.2012.01557.x

APA

Togsverd-Bo, K., Idorn, L. W., Philipsen, P. A., Wulf, H. C., & Hædersdal, M. (2012). Protoporphyrin IX formation and photobleaching in different layers of normal human skin: methyl- and hexylaminolevulinate and different light sources. Experimental Dermatology Online, 21(10), 745-750. https://doi.org/10.1111/j.1600-0625.2012.01557.x

Vancouver

Togsverd-Bo K, Idorn LW, Philipsen PA, Wulf HC, Hædersdal M. Protoporphyrin IX formation and photobleaching in different layers of normal human skin: methyl- and hexylaminolevulinate and different light sources. Experimental Dermatology Online. 2012;21(10):745-750. https://doi.org/10.1111/j.1600-0625.2012.01557.x

Author

Togsverd-Bo, Katrine ; Idorn, Luise W ; Philipsen, Peter A ; Wulf, Hans Christian ; Hædersdal, Merete. / Protoporphyrin IX formation and photobleaching in different layers of normal human skin : methyl- and hexylaminolevulinate and different light sources. I: Experimental Dermatology Online. 2012 ; Bind 21, Nr. 10. s. 745-750.

Bibtex

@article{bda129ed51784416aa83d30b90909310,
title = "Protoporphyrin IX formation and photobleaching in different layers of normal human skin: methyl- and hexylaminolevulinate and different light sources",
abstract = "Topical photodynamic therapy (PDT) is used for various skin disorders, and selective targeting of specific skin structures is desirable. The objective was to assess accumulation of PpIX fluorescence and photobleaching within skin layers using different photosensitizers and light sources. Normal human skin was tape-stripped and incubated with 20% methylaminolevulinate (MAL) or 20% hexylaminolevulinate (HAL) for 3 h. Fluorescence microscopy quantified PpIX accumulation in epidermis, superficial, mid and deep dermis, down to 2 mm. PpIX photobleaching by light-emitting diode (LED, 632 nm, 18 and 37 J/cm(2)), intense pulsed light (IPL, 500-650 nm, 36 and 72 J/cm(2)) and long-pulsed dye laser (LPDL, 595 nm, 7.5 and 15 J/cm(2)) was measured using fluorescence photography and microscopy. We found higher PpIX fluorescence intensities in epidermis and superficial dermis in HAL-incubated skin than MAL-incubated skin (P ",
author = "Katrine Togsverd-Bo and Idorn, {Luise W} and Philipsen, {Peter A} and Wulf, {Hans Christian} and Merete H{\ae}dersdal",
note = "{\textcopyright} 2012 John Wiley & Sons A/S.",
year = "2012",
doi = "10.1111/j.1600-0625.2012.01557.x",
language = "English",
volume = "21",
pages = "745--750",
journal = "Experimental Dermatology",
issn = "1600-0625",
publisher = "Wiley",
number = "10",

}

RIS

TY - JOUR

T1 - Protoporphyrin IX formation and photobleaching in different layers of normal human skin

T2 - methyl- and hexylaminolevulinate and different light sources

AU - Togsverd-Bo, Katrine

AU - Idorn, Luise W

AU - Philipsen, Peter A

AU - Wulf, Hans Christian

AU - Hædersdal, Merete

N1 - © 2012 John Wiley & Sons A/S.

PY - 2012

Y1 - 2012

N2 - Topical photodynamic therapy (PDT) is used for various skin disorders, and selective targeting of specific skin structures is desirable. The objective was to assess accumulation of PpIX fluorescence and photobleaching within skin layers using different photosensitizers and light sources. Normal human skin was tape-stripped and incubated with 20% methylaminolevulinate (MAL) or 20% hexylaminolevulinate (HAL) for 3 h. Fluorescence microscopy quantified PpIX accumulation in epidermis, superficial, mid and deep dermis, down to 2 mm. PpIX photobleaching by light-emitting diode (LED, 632 nm, 18 and 37 J/cm(2)), intense pulsed light (IPL, 500-650 nm, 36 and 72 J/cm(2)) and long-pulsed dye laser (LPDL, 595 nm, 7.5 and 15 J/cm(2)) was measured using fluorescence photography and microscopy. We found higher PpIX fluorescence intensities in epidermis and superficial dermis in HAL-incubated skin than MAL-incubated skin (P 

AB - Topical photodynamic therapy (PDT) is used for various skin disorders, and selective targeting of specific skin structures is desirable. The objective was to assess accumulation of PpIX fluorescence and photobleaching within skin layers using different photosensitizers and light sources. Normal human skin was tape-stripped and incubated with 20% methylaminolevulinate (MAL) or 20% hexylaminolevulinate (HAL) for 3 h. Fluorescence microscopy quantified PpIX accumulation in epidermis, superficial, mid and deep dermis, down to 2 mm. PpIX photobleaching by light-emitting diode (LED, 632 nm, 18 and 37 J/cm(2)), intense pulsed light (IPL, 500-650 nm, 36 and 72 J/cm(2)) and long-pulsed dye laser (LPDL, 595 nm, 7.5 and 15 J/cm(2)) was measured using fluorescence photography and microscopy. We found higher PpIX fluorescence intensities in epidermis and superficial dermis in HAL-incubated skin than MAL-incubated skin (P 

U2 - 10.1111/j.1600-0625.2012.01557.x

DO - 10.1111/j.1600-0625.2012.01557.x

M3 - Journal article

C2 - 22882358

VL - 21

SP - 745

EP - 750

JO - Experimental Dermatology

JF - Experimental Dermatology

SN - 1600-0625

IS - 10

ER -

ID: 48466151