Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics
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Drug sensitivity data acquired from solid tumor-derived cultures are often unsuitable for personalized treatment guidance due to the lengthy turnaround time. Here, we present a protocol for determining ex vivo drug sensitivities using fresh uncultured human lung tumor-derived EpCAM+ epithelial cells (FUTCs). We describe steps for drug testing in FUTCs to identify tumor cell-selective single or combination therapy in 72 h of sample processing. The FUTC-based approach can also be used to predict in vivo resistance to known targeted therapies. For complete details on the use and execution of this protocol, please refer to Talwelkar et al. (2021).
Originalsprog | Engelsk |
---|---|
Artikelnummer | 101720 |
Tidsskrift | STAR Protocols |
Vol/bind | 3 |
Udgave nummer | 4 |
Antal sider | 19 |
ISSN | 2666-1667 |
DOI | |
Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:
All the figures presented here were created with BioRender.com . The authors would like to thank the FIMM Technology Centre supported by HiLIFE and Biocenter Finland for providing the drug screening plates and services via the High-Throughput Biomediciine Unit, for Sequencing Core Unit services, and FIMM Digital Microscopy and Molecular Pathology services. In addition, we thank the HUS Diagnostic Center at HUSLAB, and thoracic pathologists at HUCH. The study received funding from the University of Helsinki Integrative Life Science doctoral program (S.S.T.); the Ida Montinin Foundation (S.S.T.); the Väinö and Laina Kivi Foundation (S.S.T.); HUSLAB and the Finnish Medical Foundation (M.I.M.); the Academy of Finland (E.W.V.; grants 307111 and 328473 ); the Novo Nordisk Foundation (K.W.; the Novo Nordisk Foundation Center for Stem Cell Biology, DanStem; grant no. NNF17CC0027852 ); HiPOC 2020 grant from the University of Helsinki (E.W.V.); and Liv och Hälsa r.f. foundation (E.W.V.). The Verschuren lab receives funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 859860 and the iCAN Digital Precision Cancer Medicine Flagship, Helsinki, Finland.
Funding Information:
All the figures presented here were created with BioRender.com. The authors would like to thank the FIMM Technology Centre supported by HiLIFE and Biocenter Finland for providing the drug screening plates and services via the High-Throughput Biomediciine Unit, for Sequencing Core Unit services, and FIMM Digital Microscopy and Molecular Pathology services. In addition, we thank the HUS Diagnostic Center at HUSLAB, and thoracic pathologists at HUCH. The study received funding from the University of Helsinki Integrative Life Science doctoral program (S.S.T.); the Ida Montinin Foundation (S.S.T.); the Väinö and Laina Kivi Foundation (S.S.T.); HUSLAB and the Finnish Medical Foundation (M.I.M.); the Academy of Finland (E.W.V.; grants 307111 and 328473); the Novo Nordisk Foundation (K.W.; the Novo Nordisk Foundation Center for Stem Cell Biology, DanStem; grant no. NNF17CC0027852); HiPOC 2020 grant from the University of Helsinki (E.W.V.); and Liv och Hälsa r.f. foundation (E.W.V.). The Verschuren lab receives funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 859860 and the iCAN Digital Precision Cancer Medicine Flagship, Helsinki, Finland. Conceptualization, S.S.T. K.W. and E.W.V.; investigation, S.S.T. M.I.M. A.H. N.L. J.R. and A.K.; writing – original draft, S.S.T. I.A.K.L. K.W. and E.W.V.; writing – review & editing, S.S.T. I.A.K.L. M.I.M. K.W. and E.W.V.; funding acquisition, S.S.T, I.A.K.L. M.I.M. K.W. and E.W.V.; supervision, K.W. and E.W.V. The authors declare no competing interests.
Publisher Copyright:
© 2022 The Authors
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