Prolyl hydroxylation in elastin is not random
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Prolyl hydroxylation in elastin is not random. / Schmelzer, Christian E H; Nagel, Marcus B M; Dziomba, Szymon; Merkher, Yulia; Sivan, Sarit S; Heinz, Andrea.
I: B B A - Reviews on Cancer, Bind 1860, Nr. 10, 10.2016, s. 2169-77.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Prolyl hydroxylation in elastin is not random
AU - Schmelzer, Christian E H
AU - Nagel, Marcus B M
AU - Dziomba, Szymon
AU - Merkher, Yulia
AU - Sivan, Sarit S
AU - Heinz, Andrea
N1 - Copyright © 2016 Elsevier B.V. All rights reserved.
PY - 2016/10
Y1 - 2016/10
N2 - BACKGROUND: This study aimed to investigate the prolyl and lysine hydroxylation in elastin from different species and tissues.METHODS: Enzymatic digests of elastin samples from human, cattle, pig and chicken were analyzed using mass spectrometry and bioinformatics tools.RESULTS: It was confirmed at the protein level that elastin does not contain hydroxylated lysine residues regardless of the species. In contrast, prolyl hydroxylation sites were identified in all elastin samples. Moreover, the analysis of the residues adjacent to prolines allowed the determination of the substrate site preferences of prolyl 4-hydroxylase. It was found that elastins from all analyzed species contain hydroxyproline and that at least 20%-24% of all proline residues were partially hydroxylated. Determination of the hydroxylation degrees of specific proline residues revealed that prolyl hydroxylation depends on both the species and the tissue, however, is independent of age. The fact that the highest hydroxylation degrees of proline residues were found for elastin from the intervertebral disc and knowledge of elastin arrangement in this tissue suggest that hydroxylation plays a biomechanical role. Interestingly, a proline-rich domain of tropoelastin (domain 24), which contains several repeats of bioactive motifs, does not show any hydroxyproline residues in the mammals studied.CONCLUSIONS: The results show that prolyl hydroxylation is not a coincidental feature and may contribute to the adaptation of the properties of elastin to meet the functional requirements of different tissues.GENERAL SIGNIFICANCE: The study for the first time shows that prolyl hydroxylation is highly regulated in elastin.
AB - BACKGROUND: This study aimed to investigate the prolyl and lysine hydroxylation in elastin from different species and tissues.METHODS: Enzymatic digests of elastin samples from human, cattle, pig and chicken were analyzed using mass spectrometry and bioinformatics tools.RESULTS: It was confirmed at the protein level that elastin does not contain hydroxylated lysine residues regardless of the species. In contrast, prolyl hydroxylation sites were identified in all elastin samples. Moreover, the analysis of the residues adjacent to prolines allowed the determination of the substrate site preferences of prolyl 4-hydroxylase. It was found that elastins from all analyzed species contain hydroxyproline and that at least 20%-24% of all proline residues were partially hydroxylated. Determination of the hydroxylation degrees of specific proline residues revealed that prolyl hydroxylation depends on both the species and the tissue, however, is independent of age. The fact that the highest hydroxylation degrees of proline residues were found for elastin from the intervertebral disc and knowledge of elastin arrangement in this tissue suggest that hydroxylation plays a biomechanical role. Interestingly, a proline-rich domain of tropoelastin (domain 24), which contains several repeats of bioactive motifs, does not show any hydroxyproline residues in the mammals studied.CONCLUSIONS: The results show that prolyl hydroxylation is not a coincidental feature and may contribute to the adaptation of the properties of elastin to meet the functional requirements of different tissues.GENERAL SIGNIFICANCE: The study for the first time shows that prolyl hydroxylation is highly regulated in elastin.
KW - Animals
KW - Cattle
KW - Chickens
KW - Collagen
KW - Elastin
KW - Humans
KW - Hydroxylation
KW - Lysine
KW - Organ Specificity
KW - Proline
KW - Prolyl Hydroxylases
KW - Protein Processing, Post-Translational
KW - Swine
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1016/j.bbagen.2016.05.013
DO - 10.1016/j.bbagen.2016.05.013
M3 - Journal article
C2 - 27180175
VL - 1860
SP - 2169
EP - 2177
JO - Biochimica et Biophysica Acta - Reviews on Cancer
JF - Biochimica et Biophysica Acta - Reviews on Cancer
SN - 0304-419X
IS - 10
ER -
ID: 186421744