Plasma Membrane Wounding and Repair Assays for Eukaryotic Cells

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Standard

Plasma Membrane Wounding and Repair Assays for Eukaryotic Cells. / Sønder, Stine Lauritzen; Ebstrup, Malene Laage; Dias, Catarina; Busk Heitmann, Anne Sofie; Nylandsted, Jesper.

I: Bio-protocol, Bind 12, Nr. 11, e4437, 2022.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Sønder, SL, Ebstrup, ML, Dias, C, Busk Heitmann, AS & Nylandsted, J 2022, 'Plasma Membrane Wounding and Repair Assays for Eukaryotic Cells', Bio-protocol, bind 12, nr. 11, e4437. https://doi.org/10.21769/BioProtoc.4437

APA

Sønder, S. L., Ebstrup, M. L., Dias, C., Busk Heitmann, A. S., & Nylandsted, J. (2022). Plasma Membrane Wounding and Repair Assays for Eukaryotic Cells. Bio-protocol, 12(11), [e4437]. https://doi.org/10.21769/BioProtoc.4437

Vancouver

Sønder SL, Ebstrup ML, Dias C, Busk Heitmann AS, Nylandsted J. Plasma Membrane Wounding and Repair Assays for Eukaryotic Cells. Bio-protocol. 2022;12(11). e4437. https://doi.org/10.21769/BioProtoc.4437

Author

Sønder, Stine Lauritzen ; Ebstrup, Malene Laage ; Dias, Catarina ; Busk Heitmann, Anne Sofie ; Nylandsted, Jesper. / Plasma Membrane Wounding and Repair Assays for Eukaryotic Cells. I: Bio-protocol. 2022 ; Bind 12, Nr. 11.

Bibtex

@article{b70ae70c44b64871935cc6cfc49a9e7d,

title = "Plasma Membrane Wounding and Repair Assays for Eukaryotic Cells",

abstract = "Damage to the plasma membrane and loss of membrane integrity are detrimental to eukaryotic cells. It is, therefore, essential that cells possess an efficient membrane repair system to survive. However, the different cellular and molecular mechanisms behind plasma membrane repair have not been fully elucidated. Here, we present three complementary methods for plasma membrane wounding, and measurement of membrane repair and integrity. The first protocol is based on real time imaging of cell membrane repair kinetics in response to laser-induced injury. The second and third protocols are end point assays that provide a population-based measure of membrane integrity, after either mechanical injury by vortex mixing with glass beads, or by detergent-induced injury by digitonin in sublytic concentrations. The protocols can be applied to most adherent eukaryotic cells in culture, as well as cells in suspension.",

keywords = "Cell injury, Imaging, Membrane integrity, Membrane wounding, Plasma membrane damage, Plasma membrane repair assays",

author = "S{\o}nder, {Stine Lauritzen} and Ebstrup, {Malene Laage} and Catarina Dias and {Busk Heitmann}, {Anne Sofie} and Jesper Nylandsted",

year = "2022",

doi = "10.21769/BioProtoc.4437",

language = "English",

volume = "12",

journal = "Bio-protocol",

issn = "2331-8325",

publisher = "bio-protocol",

number = "11",

}

RIS

TY - JOUR

T1 - Plasma Membrane Wounding and Repair Assays for Eukaryotic Cells

AU - Sønder, Stine Lauritzen

AU - Ebstrup, Malene Laage

AU - Dias, Catarina

AU - Busk Heitmann, Anne Sofie

AU - Nylandsted, Jesper

PY - 2022

Y1 - 2022

N2 - Damage to the plasma membrane and loss of membrane integrity are detrimental to eukaryotic cells. It is, therefore, essential that cells possess an efficient membrane repair system to survive. However, the different cellular and molecular mechanisms behind plasma membrane repair have not been fully elucidated. Here, we present three complementary methods for plasma membrane wounding, and measurement of membrane repair and integrity. The first protocol is based on real time imaging of cell membrane repair kinetics in response to laser-induced injury. The second and third protocols are end point assays that provide a population-based measure of membrane integrity, after either mechanical injury by vortex mixing with glass beads, or by detergent-induced injury by digitonin in sublytic concentrations. The protocols can be applied to most adherent eukaryotic cells in culture, as well as cells in suspension.

AB - Damage to the plasma membrane and loss of membrane integrity are detrimental to eukaryotic cells. It is, therefore, essential that cells possess an efficient membrane repair system to survive. However, the different cellular and molecular mechanisms behind plasma membrane repair have not been fully elucidated. Here, we present three complementary methods for plasma membrane wounding, and measurement of membrane repair and integrity. The first protocol is based on real time imaging of cell membrane repair kinetics in response to laser-induced injury. The second and third protocols are end point assays that provide a population-based measure of membrane integrity, after either mechanical injury by vortex mixing with glass beads, or by detergent-induced injury by digitonin in sublytic concentrations. The protocols can be applied to most adherent eukaryotic cells in culture, as well as cells in suspension.

KW - Cell injury

KW - Imaging

KW - Membrane integrity

KW - Membrane wounding

KW - Plasma membrane damage

KW - Plasma membrane repair assays

U2 - 10.21769/BioProtoc.4437

DO - 10.21769/BioProtoc.4437

M3 - Journal article

C2 - 35799909

AN - SCOPUS:85133326001

VL - 12

JO - Bio-protocol

JF - Bio-protocol

SN - 2331-8325

IS - 11

M1 - e4437

ER -

ID: 316679741