Photochemical cross-linking of protein and DNA in chromatin. Synthesis and application of a photosensitive cleavable derivative of 9-aminoacridine with two photoprobes connected through a disulphide-containing linker

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Standard

Photochemical cross-linking of protein and DNA in chromatin. Synthesis and application of a photosensitive cleavable derivative of 9-aminoacridine with two photoprobes connected through a disulphide-containing linker. / Nielsen, Peter E.; Hansen, J B; Buchardt, O.

I: Biochemical Journal, Bind 223, Nr. 2, 15.10.1984, s. 519-26.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Nielsen, PE, Hansen, JB & Buchardt, O 1984, 'Photochemical cross-linking of protein and DNA in chromatin. Synthesis and application of a photosensitive cleavable derivative of 9-aminoacridine with two photoprobes connected through a disulphide-containing linker', Biochemical Journal, bind 223, nr. 2, s. 519-26. https://doi.org/10.1042/bj2230519

APA

Nielsen, P. E., Hansen, J. B., & Buchardt, O. (1984). Photochemical cross-linking of protein and DNA in chromatin. Synthesis and application of a photosensitive cleavable derivative of 9-aminoacridine with two photoprobes connected through a disulphide-containing linker. Biochemical Journal, 223(2), 519-26. https://doi.org/10.1042/bj2230519

Vancouver

Nielsen PE, Hansen JB, Buchardt O. Photochemical cross-linking of protein and DNA in chromatin. Synthesis and application of a photosensitive cleavable derivative of 9-aminoacridine with two photoprobes connected through a disulphide-containing linker. Biochemical Journal. 1984 okt. 15;223(2):519-26. https://doi.org/10.1042/bj2230519

Author

Nielsen, Peter E. ; Hansen, J B ; Buchardt, O. / Photochemical cross-linking of protein and DNA in chromatin. Synthesis and application of a photosensitive cleavable derivative of 9-aminoacridine with two photoprobes connected through a disulphide-containing linker. I: Biochemical Journal. 1984 ; Bind 223, Nr. 2. s. 519-26.

Bibtex

@article{bcb62d646cfb4668a8289601eb067418,
title = "Photochemical cross-linking of protein and DNA in chromatin. Synthesis and application of a photosensitive cleavable derivative of 9-aminoacridine with two photoprobes connected through a disulphide-containing linker",
abstract = "A novel cleavable photo-cross-linking reagent, N-(2-methoxy-6-azidoacridin-9-yl)-N'-(4-azidobenzoyl)cystamine, for analysis of protein-nucleic acid interactions, has been synthesized. The reagent contains two photosensitive groups that can be activated sequentially. The azidoacridinyl moiety is sensitive to u.v. and visible light (lambda less than or equal to 450 nm), whereas the azidobenzoyl part needs higher-energy light (lambda less than or equal to 350 nm). Furthermore, the disulphide bridge connecting the two photoactive groups can be cleaved by reduction with mercaptans. The reagent is shown to induce cleavable cross-links between all five major histones and DNA in chromatin from Ehrlich ascites cells on activation with long-wavelength u.v. light (lambda greater than 300 nm) at an efficiency of approximately 3% of the added reagent.",
keywords = "Azides/chemical synthesis, Chromatin/analysis, DNA, Electrophoresis, Polyacrylamide Gel, Histones, Photochemistry, Spectrophotometry, Ultraviolet Rays",
author = "Nielsen, {Peter E.} and Hansen, {J B} and O Buchardt",
year = "1984",
month = oct,
day = "15",
doi = "10.1042/bj2230519",
language = "English",
volume = "223",
pages = "519--26",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd.",
number = "2",

}

RIS

TY - JOUR

T1 - Photochemical cross-linking of protein and DNA in chromatin. Synthesis and application of a photosensitive cleavable derivative of 9-aminoacridine with two photoprobes connected through a disulphide-containing linker

AU - Nielsen, Peter E.

AU - Hansen, J B

AU - Buchardt, O

PY - 1984/10/15

Y1 - 1984/10/15

N2 - A novel cleavable photo-cross-linking reagent, N-(2-methoxy-6-azidoacridin-9-yl)-N'-(4-azidobenzoyl)cystamine, for analysis of protein-nucleic acid interactions, has been synthesized. The reagent contains two photosensitive groups that can be activated sequentially. The azidoacridinyl moiety is sensitive to u.v. and visible light (lambda less than or equal to 450 nm), whereas the azidobenzoyl part needs higher-energy light (lambda less than or equal to 350 nm). Furthermore, the disulphide bridge connecting the two photoactive groups can be cleaved by reduction with mercaptans. The reagent is shown to induce cleavable cross-links between all five major histones and DNA in chromatin from Ehrlich ascites cells on activation with long-wavelength u.v. light (lambda greater than 300 nm) at an efficiency of approximately 3% of the added reagent.

AB - A novel cleavable photo-cross-linking reagent, N-(2-methoxy-6-azidoacridin-9-yl)-N'-(4-azidobenzoyl)cystamine, for analysis of protein-nucleic acid interactions, has been synthesized. The reagent contains two photosensitive groups that can be activated sequentially. The azidoacridinyl moiety is sensitive to u.v. and visible light (lambda less than or equal to 450 nm), whereas the azidobenzoyl part needs higher-energy light (lambda less than or equal to 350 nm). Furthermore, the disulphide bridge connecting the two photoactive groups can be cleaved by reduction with mercaptans. The reagent is shown to induce cleavable cross-links between all five major histones and DNA in chromatin from Ehrlich ascites cells on activation with long-wavelength u.v. light (lambda greater than 300 nm) at an efficiency of approximately 3% of the added reagent.

KW - Azides/chemical synthesis

KW - Chromatin/analysis

KW - DNA

KW - Electrophoresis, Polyacrylamide Gel

KW - Histones

KW - Photochemistry

KW - Spectrophotometry

KW - Ultraviolet Rays

U2 - 10.1042/bj2230519

DO - 10.1042/bj2230519

M3 - Journal article

C2 - 6497860

VL - 223

SP - 519

EP - 526

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 2

ER -

ID: 203631879