Phosphoproteins of the chicken eggshell calcified layer
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Phosphoproteins of the chicken eggshell calcified layer. / Mann, Karlheinz; Olsen, Jesper Velgaard; Macek, Boris; Gnad, Florian; Mann, Matthias.
I: Proteomics, Bind 7, Nr. 1, 2007, s. 106-15.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Phosphoproteins of the chicken eggshell calcified layer
AU - Mann, Karlheinz
AU - Olsen, Jesper Velgaard
AU - Macek, Boris
AU - Gnad, Florian
AU - Mann, Matthias
N1 - Keywords: Amino Acid Sequence; Animals; Chickens; Egg Shell; Mass Spectrometry; Molecular Sequence Data; Phosphoproteins; Phosphorylation
PY - 2007
Y1 - 2007
N2 - The chicken eggshell matrix is a complex mixture of proteins and proteoglycans. It also contains phosphoproteins that are thought to affect mineralization of the matrix. Several of the matrix phosphoproteins, such as the major component osteopontin, have already been identified as phosphoproteins in other tissues, but the phosphorylation status of the eggshell matrix forms was unknown. The phosphopeptides, obtained after cleavage of the matrix proteins with several different cleavage methods, were enriched by anion-exchange chromatography and reversible binding to titanium oxide and identified by LC-MS(n) or pseudo-MS(n) analysis following neutral loss scanning. Altogether we identified 39 phosphorylated matrix proteins, 22 of which were not known to be phosphorylated before. Eight of the proteins were identified as eggshell matrix components for the first time. Together these proteins contained more than 150 different phosphorylation sites, 103 of which were determined with high confidence. Among the major phosphorylated proteins of the chicken eggshell matrix were osteopontin and the eggshell-specific proteins ovocleidin-17, ovocleidin-116, and ovocalyxin-32.
AB - The chicken eggshell matrix is a complex mixture of proteins and proteoglycans. It also contains phosphoproteins that are thought to affect mineralization of the matrix. Several of the matrix phosphoproteins, such as the major component osteopontin, have already been identified as phosphoproteins in other tissues, but the phosphorylation status of the eggshell matrix forms was unknown. The phosphopeptides, obtained after cleavage of the matrix proteins with several different cleavage methods, were enriched by anion-exchange chromatography and reversible binding to titanium oxide and identified by LC-MS(n) or pseudo-MS(n) analysis following neutral loss scanning. Altogether we identified 39 phosphorylated matrix proteins, 22 of which were not known to be phosphorylated before. Eight of the proteins were identified as eggshell matrix components for the first time. Together these proteins contained more than 150 different phosphorylation sites, 103 of which were determined with high confidence. Among the major phosphorylated proteins of the chicken eggshell matrix were osteopontin and the eggshell-specific proteins ovocleidin-17, ovocleidin-116, and ovocalyxin-32.
U2 - 10.1002/pmic.200600635
DO - 10.1002/pmic.200600635
M3 - Journal article
VL - 7
SP - 106
EP - 115
JO - Proteomics
JF - Proteomics
SN - 1615-9853
IS - 1
ER -
ID: 46461635