Phage display of peptide / major histocompatibility class I complexes

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Standard

Phage display of peptide / major histocompatibility class I complexes. / Vest Hansen, N; Ostergaard Pedersen, L; Stryhn, A; Buus, S.

I: European Journal of Immunology, Bind 31, Nr. 1, 2001, s. 32-8.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Vest Hansen, N, Ostergaard Pedersen, L, Stryhn, A & Buus, S 2001, 'Phage display of peptide / major histocompatibility class I complexes', European Journal of Immunology, bind 31, nr. 1, s. 32-8.

APA

Vest Hansen, N., Ostergaard Pedersen, L., Stryhn, A., & Buus, S. (2001). Phage display of peptide / major histocompatibility class I complexes. European Journal of Immunology, 31(1), 32-8.

Vancouver

Vest Hansen N, Ostergaard Pedersen L, Stryhn A, Buus S. Phage display of peptide / major histocompatibility class I complexes. European Journal of Immunology. 2001;31(1):32-8.

Author

Vest Hansen, N ; Ostergaard Pedersen, L ; Stryhn, A ; Buus, S. / Phage display of peptide / major histocompatibility class I complexes. I: European Journal of Immunology. 2001 ; Bind 31, Nr. 1. s. 32-8.

Bibtex

@article{0650fe90ebcc11ddbf70000ea68e967b,
title = "Phage display of peptide / major histocompatibility class I complexes",
abstract = "Major histocompatibility complex class I (MHC-I) molecules sample peptides from the intracellular environment and present them to cytotoxic T cells (CTL). To establish a selection system, and, thereby, enable a library approach to identify the specificities involved (that of the MHC-I for peptides and subsequently that ot the T cell receptor for peptide-MHC-I complex), we have fused a single chain peptide-MHC-I complex to the phage minor coat protein, gpIII, and displayed it on filamentous phage. Expression of peptide-MHC-I complexes was shown with relevant conformation-specific monoclonal antibodies and, more importantly, with a unique {"}T cell receptor-like{"} (i. e. peptide-specific, MHC-I-restricted) antibody. Thus, properly assembled and folded peptide-MHC-I complexes can be displayed on filamentous phage. Despite the successful display, interaction with T cells could not be demonstrated.",
author = "{Vest Hansen}, N and {Ostergaard Pedersen}, L and A Stryhn and S Buus",
note = "Keywords: Amino Acid Sequence; Bacteriophages; Base Sequence; Histocompatibility Antigens Class I; Molecular Sequence Data; Peptides; Protein Folding; Receptors, Antigen, T-Cell; Recombinant Fusion Proteins",
year = "2001",
language = "English",
volume = "31",
pages = "32--8",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "1",

}

RIS

TY - JOUR

T1 - Phage display of peptide / major histocompatibility class I complexes

AU - Vest Hansen, N

AU - Ostergaard Pedersen, L

AU - Stryhn, A

AU - Buus, S

N1 - Keywords: Amino Acid Sequence; Bacteriophages; Base Sequence; Histocompatibility Antigens Class I; Molecular Sequence Data; Peptides; Protein Folding; Receptors, Antigen, T-Cell; Recombinant Fusion Proteins

PY - 2001

Y1 - 2001

N2 - Major histocompatibility complex class I (MHC-I) molecules sample peptides from the intracellular environment and present them to cytotoxic T cells (CTL). To establish a selection system, and, thereby, enable a library approach to identify the specificities involved (that of the MHC-I for peptides and subsequently that ot the T cell receptor for peptide-MHC-I complex), we have fused a single chain peptide-MHC-I complex to the phage minor coat protein, gpIII, and displayed it on filamentous phage. Expression of peptide-MHC-I complexes was shown with relevant conformation-specific monoclonal antibodies and, more importantly, with a unique "T cell receptor-like" (i. e. peptide-specific, MHC-I-restricted) antibody. Thus, properly assembled and folded peptide-MHC-I complexes can be displayed on filamentous phage. Despite the successful display, interaction with T cells could not be demonstrated.

AB - Major histocompatibility complex class I (MHC-I) molecules sample peptides from the intracellular environment and present them to cytotoxic T cells (CTL). To establish a selection system, and, thereby, enable a library approach to identify the specificities involved (that of the MHC-I for peptides and subsequently that ot the T cell receptor for peptide-MHC-I complex), we have fused a single chain peptide-MHC-I complex to the phage minor coat protein, gpIII, and displayed it on filamentous phage. Expression of peptide-MHC-I complexes was shown with relevant conformation-specific monoclonal antibodies and, more importantly, with a unique "T cell receptor-like" (i. e. peptide-specific, MHC-I-restricted) antibody. Thus, properly assembled and folded peptide-MHC-I complexes can be displayed on filamentous phage. Despite the successful display, interaction with T cells could not be demonstrated.

M3 - Journal article

C2 - 11169435

VL - 31

SP - 32

EP - 38

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 1

ER -

ID: 9944368