Olfactomedin 4 defines a subset of human neutrophils
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Olfactomedin 4 defines a subset of human neutrophils. / Clemmensen, Stine N; Bohr, Christina T; Rørvig, Sara; Glenthøj, Andreas; Mora-Jensen, Helena; Cramer, Elisabeth P; Jacobsen, Lars C; Larsen, Maria T; Cowland, Jack B; Tanassi, Julia T; Heegaard, Niels H H; Wren, Jonathan D; Silahtaroglu, Asli N; Borregaard, Niels.
I: Journal of Leukocyte Biology, Bind 91, Nr. 3, 03.2012, s. 495-500.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Olfactomedin 4 defines a subset of human neutrophils
AU - Clemmensen, Stine N
AU - Bohr, Christina T
AU - Rørvig, Sara
AU - Glenthøj, Andreas
AU - Mora-Jensen, Helena
AU - Cramer, Elisabeth P
AU - Jacobsen, Lars C
AU - Larsen, Maria T
AU - Cowland, Jack B
AU - Tanassi, Julia T
AU - Heegaard, Niels H H
AU - Wren, Jonathan D
AU - Silahtaroglu, Asli N
AU - Borregaard, Niels
PY - 2012/3
Y1 - 2012/3
N2 - OLFM4 was identified initially as a gene highly induced in myeloid stem cells by G-CSF treatment. A bioinformatics method using a global meta-analysis of microarray data predicted that OLFM4 would be associated with specific granules in human neutrophils. Subcellular fractionation of peripheral blood neutrophils demonstrated complete colocalization of OLFM4 with the specific granule protein NGAL, and stimulation of neutrophils with PMA resulted in corelease of NGAL and OLFM4, proving that OLFM4 is a genuine constituent of neutrophil-specific granules. In accordance with this, OLFM4 mRNA peaked at the MY/MM stage of maturation. OLFM4 was, however, present in only 20-25% of peripheral blood neutrophils, as determined by immunocytochemistry and flow cytometry, whereas mRNA for OLFM4 was present in all MY/MM, indicating post-transcriptional regulation as a basis for the heterogeneous expression of OLFM4 protein.
AB - OLFM4 was identified initially as a gene highly induced in myeloid stem cells by G-CSF treatment. A bioinformatics method using a global meta-analysis of microarray data predicted that OLFM4 would be associated with specific granules in human neutrophils. Subcellular fractionation of peripheral blood neutrophils demonstrated complete colocalization of OLFM4 with the specific granule protein NGAL, and stimulation of neutrophils with PMA resulted in corelease of NGAL and OLFM4, proving that OLFM4 is a genuine constituent of neutrophil-specific granules. In accordance with this, OLFM4 mRNA peaked at the MY/MM stage of maturation. OLFM4 was, however, present in only 20-25% of peripheral blood neutrophils, as determined by immunocytochemistry and flow cytometry, whereas mRNA for OLFM4 was present in all MY/MM, indicating post-transcriptional regulation as a basis for the heterogeneous expression of OLFM4 protein.
KW - Bone Marrow Cells
KW - Cell Differentiation
KW - Granulocyte Colony-Stimulating Factor
KW - Humans
KW - Myeloid Cells
KW - Neutrophils
KW - Protein Transport
KW - RNA, Messenger
U2 - 10.1189/jlb.0811417
DO - 10.1189/jlb.0811417
M3 - Journal article
C2 - 22187488
VL - 91
SP - 495
EP - 500
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
SN - 0741-5400
IS - 3
ER -
ID: 38062653