Next-generation sequencing offers new insights into DNA degradation

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Next-generation sequencing offers new insights into DNA degradation. / Overballe-Petersen, Søren; Orlando, Ludovic Antoine Alexandre; Willerslev, Eske.

I: Trends in Biotechnology, Bind 30, Nr. 7, 2012, s. 364-368.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Overballe-Petersen, S, Orlando, LAA & Willerslev, E 2012, 'Next-generation sequencing offers new insights into DNA degradation', Trends in Biotechnology, bind 30, nr. 7, s. 364-368. https://doi.org/10.1016/j.tibtech.2012.03.007

APA

Overballe-Petersen, S., Orlando, L. A. A., & Willerslev, E. (2012). Next-generation sequencing offers new insights into DNA degradation. Trends in Biotechnology, 30(7), 364-368. https://doi.org/10.1016/j.tibtech.2012.03.007

Vancouver

Overballe-Petersen S, Orlando LAA, Willerslev E. Next-generation sequencing offers new insights into DNA degradation. Trends in Biotechnology. 2012;30(7):364-368. https://doi.org/10.1016/j.tibtech.2012.03.007

Author

Overballe-Petersen, Søren ; Orlando, Ludovic Antoine Alexandre ; Willerslev, Eske. / Next-generation sequencing offers new insights into DNA degradation. I: Trends in Biotechnology. 2012 ; Bind 30, Nr. 7. s. 364-368.

Bibtex

@article{fc43bdd21afb403cb3a2562566116618,

title = "Next-generation sequencing offers new insights into DNA degradation",

abstract = "The processes underlying DNA degradation are central to various disciplines, including cancer research, forensics and archaeology. The sequencing of ancient DNA molecules on next-generation sequencing platforms provides direct measurements of cytosine deamination, depurination and fragmentation rates that previously were obtained only from extrapolations of results from in vitro kinetic experiments performed over short timescales. For example, recent next-generation sequencing of ancient DNA reveals purine bases as one of the main targets of postmortem hydrolytic damage, through base elimination and strand breakage. It also shows substantially increased rates of DNA base-loss at guanosine. In this review, we argue that the latter results from an electron resonance structure unique to guanosine rather than adenosine having an extra resonance structure over guanosine as previously suggested.",

keywords = "Animals, DNA, DNA Damage, Fossils, Horses, Humans, Mammals, Models, Genetic, Nucleic Acid Conformation, Sequence Analysis, DNA",

author = "S{\o}ren Overballe-Petersen and Orlando, {Ludovic Antoine Alexandre} and Eske Willerslev",

year = "2012",

doi = "10.1016/j.tibtech.2012.03.007",

language = "English",

volume = "30",

pages = "364--368",

journal = "Trends in Biotechnology",

issn = "0167-7799",

publisher = "Elsevier Ltd. * Trends Journals",

number = "7",

}

RIS

TY - JOUR

T1 - Next-generation sequencing offers new insights into DNA degradation

AU - Overballe-Petersen, Søren

AU - Orlando, Ludovic Antoine Alexandre

AU - Willerslev, Eske

PY - 2012

Y1 - 2012

N2 - The processes underlying DNA degradation are central to various disciplines, including cancer research, forensics and archaeology. The sequencing of ancient DNA molecules on next-generation sequencing platforms provides direct measurements of cytosine deamination, depurination and fragmentation rates that previously were obtained only from extrapolations of results from in vitro kinetic experiments performed over short timescales. For example, recent next-generation sequencing of ancient DNA reveals purine bases as one of the main targets of postmortem hydrolytic damage, through base elimination and strand breakage. It also shows substantially increased rates of DNA base-loss at guanosine. In this review, we argue that the latter results from an electron resonance structure unique to guanosine rather than adenosine having an extra resonance structure over guanosine as previously suggested.

AB - The processes underlying DNA degradation are central to various disciplines, including cancer research, forensics and archaeology. The sequencing of ancient DNA molecules on next-generation sequencing platforms provides direct measurements of cytosine deamination, depurination and fragmentation rates that previously were obtained only from extrapolations of results from in vitro kinetic experiments performed over short timescales. For example, recent next-generation sequencing of ancient DNA reveals purine bases as one of the main targets of postmortem hydrolytic damage, through base elimination and strand breakage. It also shows substantially increased rates of DNA base-loss at guanosine. In this review, we argue that the latter results from an electron resonance structure unique to guanosine rather than adenosine having an extra resonance structure over guanosine as previously suggested.

KW - Animals

KW - DNA

KW - DNA Damage

KW - Fossils

KW - Horses

KW - Humans

KW - Mammals

KW - Models, Genetic

KW - Nucleic Acid Conformation

KW - Sequence Analysis, DNA

U2 - 10.1016/j.tibtech.2012.03.007

DO - 10.1016/j.tibtech.2012.03.007

M3 - Journal article

C2 - 22516743

VL - 30

SP - 364

EP - 368

JO - Trends in Biotechnology

JF - Trends in Biotechnology

SN - 0167-7799

IS - 7

ER -

ID: 49691278