Nephron blood flow dynamics measured by laser speckle contrast imaging
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Nephron blood flow dynamics measured by laser speckle contrast imaging. / von Holstein-Rathlou, Niels-Henrik; Sosnovtseva, Olga V; Pavlov, Alexey N; Cupples, William A; Sørensen, Charlotte Mehlin; Marsh, Donald J.
I: American Journal of Physiology: Renal Physiology, Bind 300, Nr. 2, 01.02.2011, s. F319-29.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Nephron blood flow dynamics measured by laser speckle contrast imaging
AU - von Holstein-Rathlou, Niels-Henrik
AU - Sosnovtseva, Olga V
AU - Pavlov, Alexey N
AU - Cupples, William A
AU - Sørensen, Charlotte Mehlin
AU - Marsh, Donald J
PY - 2011/2/1
Y1 - 2011/2/1
N2 - Tubuloglomerular feedback (TGF) has an important role in autoregulation of renal blood flow and glomerular filtration rate (GFR). Because of the characteristics of signal transmission in the feedback loop, the TGF undergoes self-sustained oscillations in single-nephron blood flow, GFR, and tubular pressure and flow. Nephrons interact by exchanging electrical signals conducted electrotonically through cells of the vascular wall, leading to synchronization of the TGF-mediated oscillations. Experimental studies of these interactions have been limited to observations on two or at most three nephrons simultaneously. The interacting nephron fields are likely to be more extensive. We have turned to laser speckle contrast imaging to measure the blood flow dynamics of 50-100 nephrons simultaneously on the renal surface of anesthetized rats. We report the application of this method and describe analytic techniques for extracting the desired data and for examining them for evidence of nephron synchronization. Synchronized TGF oscillations were detected in pairs or triplets of nephrons. The amplitude and the frequency of the oscillations changed with time, as did the patterns of synchronization. Synchronization may take place among nephrons not immediately adjacent on the surface of the kidney.
AB - Tubuloglomerular feedback (TGF) has an important role in autoregulation of renal blood flow and glomerular filtration rate (GFR). Because of the characteristics of signal transmission in the feedback loop, the TGF undergoes self-sustained oscillations in single-nephron blood flow, GFR, and tubular pressure and flow. Nephrons interact by exchanging electrical signals conducted electrotonically through cells of the vascular wall, leading to synchronization of the TGF-mediated oscillations. Experimental studies of these interactions have been limited to observations on two or at most three nephrons simultaneously. The interacting nephron fields are likely to be more extensive. We have turned to laser speckle contrast imaging to measure the blood flow dynamics of 50-100 nephrons simultaneously on the renal surface of anesthetized rats. We report the application of this method and describe analytic techniques for extracting the desired data and for examining them for evidence of nephron synchronization. Synchronized TGF oscillations were detected in pairs or triplets of nephrons. The amplitude and the frequency of the oscillations changed with time, as did the patterns of synchronization. Synchronization may take place among nephrons not immediately adjacent on the surface of the kidney.
KW - Acetylcholine
KW - Angiotensin II
KW - Animals
KW - Hemodynamics
KW - Lasers
KW - Male
KW - Nephrons
KW - Rats
KW - Rats, Sprague-Dawley
KW - Renal Circulation
KW - Rheology
U2 - 10.1152/ajprenal.00417.2010
DO - 10.1152/ajprenal.00417.2010
M3 - Journal article
C2 - 21048025
VL - 300
SP - F319-29
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
SN - 1931-857X
IS - 2
ER -
ID: 33720315