NBR1-mediated selective autophagy of ARF7 modulates root branching

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Auxin dictates root architecture via the Auxin Response Factor (ARF) family of transcription factors, which control lateral root (LR) formation. In Arabidopsis, ARF7 regulates the specification of prebranch sites (PBS) generating LRs through gene expression oscillations and plays a pivotal role during LR initiation. Despite the importance of ARF7 in this process, there is a surprising lack of knowledge about how ARF7 turnover is regulated and how this impacts root architecture. Here, we show that ARF7 accumulates in autophagy mutants and is degraded through NBR1-dependent selective autophagy. We demonstrate that the previously reported rhythmic changes to ARF7 abundance in roots are modulated via autophagy and might occur in other tissues. In addition, we show that the level of co-localization between ARF7 and autophagy markers oscillates and can be modulated by auxin to trigger ARF7 turnover. Furthermore, we observe that autophagy impairment prevents ARF7 oscillation and reduces both PBS establishment and LR formation. In conclusion, we report a novel role for autophagy during development, namely by enacting auxin-induced selective degradation of ARF7 to optimize periodic root branching.
OriginalsprogEngelsk
TidsskriftEMBO Reports
Vol/bind25
Sider (fra-til)2571 - 2591
Antal sider21
ISSN1469-221X
DOI
StatusUdgivet - 2024

Bibliografisk note

Funding Information:
This work was funded by a Danmarks Frie Forskningsfond grant to E.R. (DFF1- 1032-00249B) and by Ministerio de Ciencia e Inovacion, Agencia Estatal de Investigaci\u00F3n (MICIN/AEI/10.13039/501100011033) of Spain, ERDF a way of making Europe and NextGenerationEU/PRTR to MAM-R (TED2021-129530B-I00 and PID2022-140719NB-I00). We would like to thank Tom Guilfoyle (anti-ARF7 antibody), Koki Yoshimoto (anti-ATG8a antibody), Malcolm Bennett (pARF7::gARF7-Venus), Liwen Jiang (NBR1-C1) and Hidehiro Fukaki (pARF19::ARF19-GFP nph4-1 arf19-1) for sharing material/reagents. We would also like to thank Suksawad Vongvisuttikun for technical assistance, the 2022 classes from the courses \u201CPlant Molecular Biology\u201D & \u201CExperimental Higher Model Organisms\u201D (University of Copenhagen, Denmark) for performing the ARF19-GFP & ARF7-Venus western blots, respectively. Confocal microscopy imaging was performed using equipment from the Center for Advanced Bioimaging (CAB) Denmark, University of Copenhagen. Mass spectrometry analyses were performed by the Proteomics Research Infrastructure (PRI) at the University of Copenhagen (UCPH), supported by the Novo Nordisk Foundation (NNF) (grant agreement number NNF19SA0059305).

Publisher Copyright:
© The Author(s) 2024.

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