TY - JOUR

T1 - Mutation screening of the glutamate cysteine ligase modifier (GCLM) gene in patients with schizophrenia

AU - Butticaz, Christophe

AU - Werge, Thomas

AU - Beckmann, Jacques S

AU - Cuénod, Michel

AU - Do, Kim Q

AU - Rivolta, Carlo

AU - Butticaz, Christophe

AU - Werge, Thomas

AU - Beckmann, Jacques S

AU - Cuénod, Michel

AU - Do, Kim Q

AU - Rivolta, Carlo

N1 - Keywords: Adult; DNA Mutational Analysis; Denmark; Female; Gene Frequency; Genetic Testing; Glutamate-Cysteine Ligase; Haplotypes; Humans; Linkage Disequilibrium; Male; Mutation; Polymorphism, Single Nucleotide; Protein Subunits; Schizophrenia

PY - 2009

Y1 - 2009

N2 - BACKGROUND: Experimental evidences show that glutathione and its rate-limiting synthesizing enzyme, the glutamate-cysteine ligase (GCL), are involved in the pathogenesis of schizophrenia. Furthermore, genetic association has been previously reported between two single nucleotide polymorphisms lying in noncoding regions of glutamate cysteine ligase modifier (GCLM) gene, which specifies for the modifier subunit of GCL and schizophrenia. OBJECTIVE: We wanted to investigate the presence of GCLM true functional mutations, likely in linkage disequilibrium with the previously identified single nucleotide polymorphism alleles, in the same set of cases that allowed the detection of the original association signal. METHODS: We screened all the coding regions of GCLM and their intronic flanking vicinities in 353 patients with schizophrenia by direct DNA sequencing. RESULTS: Ten sequence variations were identified, five of which were not previously described. None of these DNA changes was within the GCLM coding sequence and in-silico analysis failed to indicate functional impairment induced by these variations. Furthermore, screening of normal controls and downstream statistical analyses revealed no significant relationship of any of these DNA variants with schizophrenia. CONCLUSION: It is unlikely that functional mutations in the GCLM gene could play a major role in genetic predisposition to schizophrenia and further studies will be required to assess its etiological function in the disease.

AB - BACKGROUND: Experimental evidences show that glutathione and its rate-limiting synthesizing enzyme, the glutamate-cysteine ligase (GCL), are involved in the pathogenesis of schizophrenia. Furthermore, genetic association has been previously reported between two single nucleotide polymorphisms lying in noncoding regions of glutamate cysteine ligase modifier (GCLM) gene, which specifies for the modifier subunit of GCL and schizophrenia. OBJECTIVE: We wanted to investigate the presence of GCLM true functional mutations, likely in linkage disequilibrium with the previously identified single nucleotide polymorphism alleles, in the same set of cases that allowed the detection of the original association signal. METHODS: We screened all the coding regions of GCLM and their intronic flanking vicinities in 353 patients with schizophrenia by direct DNA sequencing. RESULTS: Ten sequence variations were identified, five of which were not previously described. None of these DNA changes was within the GCLM coding sequence and in-silico analysis failed to indicate functional impairment induced by these variations. Furthermore, screening of normal controls and downstream statistical analyses revealed no significant relationship of any of these DNA variants with schizophrenia. CONCLUSION: It is unlikely that functional mutations in the GCLM gene could play a major role in genetic predisposition to schizophrenia and further studies will be required to assess its etiological function in the disease.

U2 - 10.1097/YPG.0b013e32832cef21

DO - 10.1097/YPG.0b013e32832cef21

M3 - Journal article

C2 - 19455074

VL - 19

SP - 201

EP - 208

JO - Psychiatric Genetics

JF - Psychiatric Genetics

SN - 0955-8829

IS - 4

ER -