mRNA expression of 5-hydroxytryptamine 1B, 1D, and 1F receptors and their role in controlling the release of calcitonin gene-related peptide in the rat trigeminovascular system

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Standard

mRNA expression of 5-hydroxytryptamine 1B, 1D, and 1F receptors and their role in controlling the release of calcitonin gene-related peptide in the rat trigeminovascular system. / Amrutkar, Dipak V; Ploug, Kenneth B; Hay-Schmidt, Anders; Porreca, Frank; Olesen, Jes; Jansen-Olesen, Inger.

I: Pain, Bind 153, Nr. 4, 04.2012, s. 830-8.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Amrutkar, DV, Ploug, KB, Hay-Schmidt, A, Porreca, F, Olesen, J & Jansen-Olesen, I 2012, 'mRNA expression of 5-hydroxytryptamine 1B, 1D, and 1F receptors and their role in controlling the release of calcitonin gene-related peptide in the rat trigeminovascular system', Pain, bind 153, nr. 4, s. 830-8. https://doi.org/10.1016/j.pain.2012.01.005

APA

Amrutkar, D. V., Ploug, K. B., Hay-Schmidt, A., Porreca, F., Olesen, J., & Jansen-Olesen, I. (2012). mRNA expression of 5-hydroxytryptamine 1B, 1D, and 1F receptors and their role in controlling the release of calcitonin gene-related peptide in the rat trigeminovascular system. Pain, 153(4), 830-8. https://doi.org/10.1016/j.pain.2012.01.005

Vancouver

Amrutkar DV, Ploug KB, Hay-Schmidt A, Porreca F, Olesen J, Jansen-Olesen I. mRNA expression of 5-hydroxytryptamine 1B, 1D, and 1F receptors and their role in controlling the release of calcitonin gene-related peptide in the rat trigeminovascular system. Pain. 2012 apr.;153(4):830-8. https://doi.org/10.1016/j.pain.2012.01.005

Author

Amrutkar, Dipak V ; Ploug, Kenneth B ; Hay-Schmidt, Anders ; Porreca, Frank ; Olesen, Jes ; Jansen-Olesen, Inger. / mRNA expression of 5-hydroxytryptamine 1B, 1D, and 1F receptors and their role in controlling the release of calcitonin gene-related peptide in the rat trigeminovascular system. I: Pain. 2012 ; Bind 153, Nr. 4. s. 830-8.

Bibtex

@article{3907e021276642129339af3332258576,

title = "mRNA expression of 5-hydroxytryptamine 1B, 1D, and 1F receptors and their role in controlling the release of calcitonin gene-related peptide in the rat trigeminovascular system",

abstract = "Triptans, a family of 5-hydroxytryptamine (5-HT) 1B, 1D, and 1F receptor agonists, are used in the acute treatment of migraine attacks. The site of action and subtypes of the 5-HT(1) receptor that mediate the antimigraine effect have still to be identified. This study investigated the mRNA expression of these receptors and the role of 5-HT(1) receptor subtypes in controlling the release of calcitonin gene-related peptide (CGRP) in rat dura mater, trigeminal ganglion (TG), and trigeminal nucleus caudalis (TNC). The mRNA for each receptor subtype was quantified by quantitative real-time polymerase chain reaction. A high potassium concentration was used to release CGRP from dura mater, isolated TG, and TNC in vitro. The immunoreactive CGRP (iCGRP) release was measured by enzyme-linked immunoassay. The mRNA transcripts of the 3 5-HT(1) receptor subtypes were detected in the trigeminovascular system. Sumatriptan inhibited iCGRP release by 31% in dura mater, 44% in TG, and 56% in TNC. This effect was reversed by a 5-HT(1B/1D) antagonist (GR127395). The 5-HT(1F) agonist (LY-344864) was effective in the dura mater (26% iCGRP inhibition), and the 5-HT(1D) agonist (PNU-142633) had a significant effect in the TNC (48%), whereas the 5-HT(1B) agonist (CP-94253) was unable to reduce the iCGRP release in all tissues studied. We found that sumatriptan reduced the iCGRP release via activation of 5-HT(1D) and 5-HT(1F) receptor subtypes. The 5-HT(1F) receptor agonist was effective only in peripheral terminals in dura mater, whereas the 5-HT(1D) agonist had a preferential effect on central terminals in the TNC.",

keywords = "Animals, Calcitonin Gene-Related Peptide, Dura Mater, Gene Expression Regulation, Male, RNA, Messenger, Rats, Rats, Sprague-Dawley, Receptor, Serotonin, 5-HT1B, Receptor, Serotonin, 5-HT1D, Receptors, Serotonin, Serotonin Receptor Agonists, Trigeminal Ganglion, Trigeminal Nuclei",

author = "Amrutkar, {Dipak V} and Ploug, {Kenneth B} and Anders Hay-Schmidt and Frank Porreca and Jes Olesen and Inger Jansen-Olesen",

year = "2012",

month = apr,

doi = "10.1016/j.pain.2012.01.005",

language = "English",

volume = "153",

pages = "830--8",

journal = "Pain",

issn = "0304-3959",

publisher = "IASP Press",

number = "4",

}

RIS

TY - JOUR

T1 - mRNA expression of 5-hydroxytryptamine 1B, 1D, and 1F receptors and their role in controlling the release of calcitonin gene-related peptide in the rat trigeminovascular system

AU - Amrutkar, Dipak V

AU - Ploug, Kenneth B

AU - Hay-Schmidt, Anders

AU - Porreca, Frank

AU - Olesen, Jes

AU - Jansen-Olesen, Inger

PY - 2012/4

Y1 - 2012/4

N2 - Triptans, a family of 5-hydroxytryptamine (5-HT) 1B, 1D, and 1F receptor agonists, are used in the acute treatment of migraine attacks. The site of action and subtypes of the 5-HT(1) receptor that mediate the antimigraine effect have still to be identified. This study investigated the mRNA expression of these receptors and the role of 5-HT(1) receptor subtypes in controlling the release of calcitonin gene-related peptide (CGRP) in rat dura mater, trigeminal ganglion (TG), and trigeminal nucleus caudalis (TNC). The mRNA for each receptor subtype was quantified by quantitative real-time polymerase chain reaction. A high potassium concentration was used to release CGRP from dura mater, isolated TG, and TNC in vitro. The immunoreactive CGRP (iCGRP) release was measured by enzyme-linked immunoassay. The mRNA transcripts of the 3 5-HT(1) receptor subtypes were detected in the trigeminovascular system. Sumatriptan inhibited iCGRP release by 31% in dura mater, 44% in TG, and 56% in TNC. This effect was reversed by a 5-HT(1B/1D) antagonist (GR127395). The 5-HT(1F) agonist (LY-344864) was effective in the dura mater (26% iCGRP inhibition), and the 5-HT(1D) agonist (PNU-142633) had a significant effect in the TNC (48%), whereas the 5-HT(1B) agonist (CP-94253) was unable to reduce the iCGRP release in all tissues studied. We found that sumatriptan reduced the iCGRP release via activation of 5-HT(1D) and 5-HT(1F) receptor subtypes. The 5-HT(1F) receptor agonist was effective only in peripheral terminals in dura mater, whereas the 5-HT(1D) agonist had a preferential effect on central terminals in the TNC.

AB - Triptans, a family of 5-hydroxytryptamine (5-HT) 1B, 1D, and 1F receptor agonists, are used in the acute treatment of migraine attacks. The site of action and subtypes of the 5-HT(1) receptor that mediate the antimigraine effect have still to be identified. This study investigated the mRNA expression of these receptors and the role of 5-HT(1) receptor subtypes in controlling the release of calcitonin gene-related peptide (CGRP) in rat dura mater, trigeminal ganglion (TG), and trigeminal nucleus caudalis (TNC). The mRNA for each receptor subtype was quantified by quantitative real-time polymerase chain reaction. A high potassium concentration was used to release CGRP from dura mater, isolated TG, and TNC in vitro. The immunoreactive CGRP (iCGRP) release was measured by enzyme-linked immunoassay. The mRNA transcripts of the 3 5-HT(1) receptor subtypes were detected in the trigeminovascular system. Sumatriptan inhibited iCGRP release by 31% in dura mater, 44% in TG, and 56% in TNC. This effect was reversed by a 5-HT(1B/1D) antagonist (GR127395). The 5-HT(1F) agonist (LY-344864) was effective in the dura mater (26% iCGRP inhibition), and the 5-HT(1D) agonist (PNU-142633) had a significant effect in the TNC (48%), whereas the 5-HT(1B) agonist (CP-94253) was unable to reduce the iCGRP release in all tissues studied. We found that sumatriptan reduced the iCGRP release via activation of 5-HT(1D) and 5-HT(1F) receptor subtypes. The 5-HT(1F) receptor agonist was effective only in peripheral terminals in dura mater, whereas the 5-HT(1D) agonist had a preferential effect on central terminals in the TNC.

KW - Animals

KW - Calcitonin Gene-Related Peptide

KW - Dura Mater

KW - Gene Expression Regulation

KW - Male

KW - RNA, Messenger

KW - Rats

KW - Rats, Sprague-Dawley

KW - Receptor, Serotonin, 5-HT1B

KW - Receptor, Serotonin, 5-HT1D

KW - Receptors, Serotonin

KW - Serotonin Receptor Agonists

KW - Trigeminal Ganglion

KW - Trigeminal Nuclei

U2 - 10.1016/j.pain.2012.01.005

DO - 10.1016/j.pain.2012.01.005

M3 - Journal article

C2 - 22305629

VL - 153

SP - 830

EP - 838

JO - Pain

JF - Pain

SN - 0304-3959

IS - 4

ER -

ID: 47927796