Molecular properties of adult mouse gastric and intestinal epithelial progenitors in their niches
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Molecular properties of adult mouse gastric and intestinal epithelial progenitors in their niches. / Giannakis, Marios; Stappenbeck, Thaddeus S; Mills, Jason C; Leip, Douglas G; Lovett, Michael; Clifton, Sandra W; Ippolito, Joseph E; Glasscock, Jarret I; Arumugam, Manimozhiyan; Brent, Michael R; Gordon, Jeffrey I.
I: Journal of Biological Chemistry, Bind 281, Nr. 16, 2006, s. 11292-300.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Molecular properties of adult mouse gastric and intestinal epithelial progenitors in their niches
AU - Giannakis, Marios
AU - Stappenbeck, Thaddeus S
AU - Mills, Jason C
AU - Leip, Douglas G
AU - Lovett, Michael
AU - Clifton, Sandra W
AU - Ippolito, Joseph E
AU - Glasscock, Jarret I
AU - Arumugam, Manimozhiyan
AU - Brent, Michael R
AU - Gordon, Jeffrey I
PY - 2006
Y1 - 2006
N2 - We have sequenced 36,641 expressed sequence tags from laser capture microdissected adult mouse gastric and small intestinal epithelial progenitors, obtaining 4031 and 3324 unique transcripts, respectively. Using Gene Ontology (GO) terms, each data set was compared with cDNA libraries from intact adult stomach and small intestine. Genes in GO categories enriched in progenitors were filtered against genes in GO categories represented in hematopoietic, neural, and embryonic stem cell transcriptomes and mapped onto transcription factor networks, plus canonical signal transduction and metabolic pathways. Wnt/beta-catenin, phosphoinositide-3/Akt kinase, insulin-like growth factor-1, vascular endothelial growth factor, integrin, and gamma-aminobutyric acid receptor signaling cascades, plus glycerolipid, fatty acid, and amino acid metabolic pathways are among those prominently represented in adult gut progenitors. The results reveal shared as well as distinctive features of adult gut stem cells when compared with other stem cell populations.
AB - We have sequenced 36,641 expressed sequence tags from laser capture microdissected adult mouse gastric and small intestinal epithelial progenitors, obtaining 4031 and 3324 unique transcripts, respectively. Using Gene Ontology (GO) terms, each data set was compared with cDNA libraries from intact adult stomach and small intestine. Genes in GO categories enriched in progenitors were filtered against genes in GO categories represented in hematopoietic, neural, and embryonic stem cell transcriptomes and mapped onto transcription factor networks, plus canonical signal transduction and metabolic pathways. Wnt/beta-catenin, phosphoinositide-3/Akt kinase, insulin-like growth factor-1, vascular endothelial growth factor, integrin, and gamma-aminobutyric acid receptor signaling cascades, plus glycerolipid, fatty acid, and amino acid metabolic pathways are among those prominently represented in adult gut progenitors. The results reveal shared as well as distinctive features of adult gut stem cells when compared with other stem cell populations.
KW - Animals
KW - Computational Biology
KW - DNA, Complementary
KW - Epithelial Cells
KW - Epithelium
KW - Expressed Sequence Tags
KW - Gastric Mucosa
KW - Gene Library
KW - Genome
KW - Hematopoietic Stem Cells
KW - Immunohistochemistry
KW - Intestine, Small
KW - Intestines
KW - Lasers
KW - Mice
KW - Models, Biological
KW - Neurons
KW - RNA, Messenger
KW - Signal Transduction
KW - Software
KW - Stem Cells
KW - Stomach
KW - Transcription Factors
U2 - 10.1074/jbc.M512118200
DO - 10.1074/jbc.M512118200
M3 - Journal article
C2 - 16464855
VL - 281
SP - 11292
EP - 11300
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 16
ER -
ID: 43976056