miR-141-3p inhibits human stromal (mesenchymal) stem cell proliferation and differentiation

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Standard

miR-141-3p inhibits human stromal (mesenchymal) stem cell proliferation and differentiation. / Qiu, Weimin; Kassem, Moustapha.

I: BBA Molecular Cell Research, Bind 1843, Nr. 9, 09.2014, s. 2114-21.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Qiu, W & Kassem, M 2014, 'miR-141-3p inhibits human stromal (mesenchymal) stem cell proliferation and differentiation', BBA Molecular Cell Research, bind 1843, nr. 9, s. 2114-21. https://doi.org/10.1016/j.bbamcr.2014.06.004

APA

Qiu, W., & Kassem, M. (2014). miR-141-3p inhibits human stromal (mesenchymal) stem cell proliferation and differentiation. BBA Molecular Cell Research, 1843(9), 2114-21. https://doi.org/10.1016/j.bbamcr.2014.06.004

Vancouver

Qiu W, Kassem M. miR-141-3p inhibits human stromal (mesenchymal) stem cell proliferation and differentiation. BBA Molecular Cell Research. 2014 sep.;1843(9):2114-21. https://doi.org/10.1016/j.bbamcr.2014.06.004

Author

Qiu, Weimin ; Kassem, Moustapha. / miR-141-3p inhibits human stromal (mesenchymal) stem cell proliferation and differentiation. I: BBA Molecular Cell Research. 2014 ; Bind 1843, Nr. 9. s. 2114-21.

Bibtex

@article{bc8783a52c774846a24ed0d562c5d988,
title = "miR-141-3p inhibits human stromal (mesenchymal) stem cell proliferation and differentiation",
abstract = "Wnt signaling determines human stromal (mesenchymal) stem cell (hMSC) differentiation fate into the osteoblast or adipocyte lineage. microRNAs (miRNAs) are small RNA molecules of 21-25 nucleotides that regulate many aspects of osteoblast biology. Thus, we examined miRNAs regulated by Wnt signaling in hMSC. We identified miRNA (miR)-141-3p as a Wnt target which in turn inhibited Wnt signaling. Moreover, miR-141-3p inhibited hMSC proliferation by arresting cells at the G1 phase of the cell cycle. miR-141-3p inhibited osteoblast differentiation of hMSC as evidenced by reduced alkaline phosphatase activity, gene expression and in vitro mineralized matrix formation. Bioinformatic studies, Western blot analysis and 3'UTR reporter assay demonstrated that cell division cycle 25A (CDC25A) is a direct target of miR-141-3p. siRNA-mediated knock-down of CDC25A inhibited hMSC proliferation and osteoblast differentiation. In summary, miR-141-3p acts as a negative regulator of hMSC proliferation and osteoblast differentiation. Targeting miR-141-3p could be used as an anabolic therapy of low bone mass diseases, e.g. osteoporosis.",
keywords = "Animals, Base Sequence, Cell Differentiation, Cell Proliferation, G1 Phase Cell Cycle Checkpoints, Gene Expression Regulation, Gene Knockdown Techniques, Humans, Mesenchymal Stromal Cells, Mice, MicroRNAs, Molecular Sequence Data, Osteoblasts, Wnt Signaling Pathway, cdc25 Phosphatases",
author = "Weimin Qiu and Moustapha Kassem",
note = "Copyright {\textcopyright} 2014 Elsevier B.V. All rights reserved.",
year = "2014",
month = sep,
doi = "10.1016/j.bbamcr.2014.06.004",
language = "English",
volume = "1843",
pages = "2114--21",
journal = "B B A - Molecular Cell Research",
issn = "0167-4889",
publisher = "Elsevier",
number = "9",

}

RIS

TY - JOUR

T1 - miR-141-3p inhibits human stromal (mesenchymal) stem cell proliferation and differentiation

AU - Qiu, Weimin

AU - Kassem, Moustapha

N1 - Copyright © 2014 Elsevier B.V. All rights reserved.

PY - 2014/9

Y1 - 2014/9

N2 - Wnt signaling determines human stromal (mesenchymal) stem cell (hMSC) differentiation fate into the osteoblast or adipocyte lineage. microRNAs (miRNAs) are small RNA molecules of 21-25 nucleotides that regulate many aspects of osteoblast biology. Thus, we examined miRNAs regulated by Wnt signaling in hMSC. We identified miRNA (miR)-141-3p as a Wnt target which in turn inhibited Wnt signaling. Moreover, miR-141-3p inhibited hMSC proliferation by arresting cells at the G1 phase of the cell cycle. miR-141-3p inhibited osteoblast differentiation of hMSC as evidenced by reduced alkaline phosphatase activity, gene expression and in vitro mineralized matrix formation. Bioinformatic studies, Western blot analysis and 3'UTR reporter assay demonstrated that cell division cycle 25A (CDC25A) is a direct target of miR-141-3p. siRNA-mediated knock-down of CDC25A inhibited hMSC proliferation and osteoblast differentiation. In summary, miR-141-3p acts as a negative regulator of hMSC proliferation and osteoblast differentiation. Targeting miR-141-3p could be used as an anabolic therapy of low bone mass diseases, e.g. osteoporosis.

AB - Wnt signaling determines human stromal (mesenchymal) stem cell (hMSC) differentiation fate into the osteoblast or adipocyte lineage. microRNAs (miRNAs) are small RNA molecules of 21-25 nucleotides that regulate many aspects of osteoblast biology. Thus, we examined miRNAs regulated by Wnt signaling in hMSC. We identified miRNA (miR)-141-3p as a Wnt target which in turn inhibited Wnt signaling. Moreover, miR-141-3p inhibited hMSC proliferation by arresting cells at the G1 phase of the cell cycle. miR-141-3p inhibited osteoblast differentiation of hMSC as evidenced by reduced alkaline phosphatase activity, gene expression and in vitro mineralized matrix formation. Bioinformatic studies, Western blot analysis and 3'UTR reporter assay demonstrated that cell division cycle 25A (CDC25A) is a direct target of miR-141-3p. siRNA-mediated knock-down of CDC25A inhibited hMSC proliferation and osteoblast differentiation. In summary, miR-141-3p acts as a negative regulator of hMSC proliferation and osteoblast differentiation. Targeting miR-141-3p could be used as an anabolic therapy of low bone mass diseases, e.g. osteoporosis.

KW - Animals

KW - Base Sequence

KW - Cell Differentiation

KW - Cell Proliferation

KW - G1 Phase Cell Cycle Checkpoints

KW - Gene Expression Regulation

KW - Gene Knockdown Techniques

KW - Humans

KW - Mesenchymal Stromal Cells

KW - Mice

KW - MicroRNAs

KW - Molecular Sequence Data

KW - Osteoblasts

KW - Wnt Signaling Pathway

KW - cdc25 Phosphatases

U2 - 10.1016/j.bbamcr.2014.06.004

DO - 10.1016/j.bbamcr.2014.06.004

M3 - Journal article

C2 - 24937190

VL - 1843

SP - 2114

EP - 2121

JO - B B A - Molecular Cell Research

JF - B B A - Molecular Cell Research

SN - 0167-4889

IS - 9

ER -

ID: 125959030