Milk lacking α-casein leads to permanent reduction in body size in mice
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Milk lacking α-casein leads to permanent reduction in body size in mice. / Kolb, Andreas F.; Huber, Reinhard C.; Lillico, Simon G.; Carlisle, Ailsa; Robinson, Claire J.; Neil, Claire; Petrie, Linda; Sorensen, Dorte B.; Olsson, I. Anna S.; Whitelaw, C. Bruce A.
I: PLoS ONE, Bind 6, Nr. 7, e21775, 26.07.2011.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Milk lacking α-casein leads to permanent reduction in body size in mice
AU - Kolb, Andreas F.
AU - Huber, Reinhard C.
AU - Lillico, Simon G.
AU - Carlisle, Ailsa
AU - Robinson, Claire J.
AU - Neil, Claire
AU - Petrie, Linda
AU - Sorensen, Dorte B.
AU - Olsson, I. Anna S.
AU - Whitelaw, C. Bruce A.
PY - 2011/7/26
Y1 - 2011/7/26
N2 - The major physiological function of milk is the transport of amino acids, carbohydrates, lipids and minerals to mammalian offspring. Caseins, the major milk proteins, are secreted in the form of a micelle consisting of protein and calcium-phosphate. We have analysed the role of the milk protein α-casein by inactivating the corresponding gene in mice. Absence of α-casein protein significantly curtails secretion of other milk proteins and calcium-phosphate, suggesting a role for α-casein in the establishment of casein micelles. In contrast, secretion of albumin, which is not synthesized in the mammary epithelium, into milk is not reduced. The absence of α-casein also significantly inhibits transcription of the other casein genes. α-Casein deficiency severely delays pup growth during lactation and results in a life-long body size reduction compared to control animals, but has only transient effects on physical and behavioural development of the pups. The data support a critical role for α-casein in casein micelle assembly. The results also confirm lactation as a critical window of metabolic programming and suggest milk protein concentration as a decisive factor in determining adult body weight.
AB - The major physiological function of milk is the transport of amino acids, carbohydrates, lipids and minerals to mammalian offspring. Caseins, the major milk proteins, are secreted in the form of a micelle consisting of protein and calcium-phosphate. We have analysed the role of the milk protein α-casein by inactivating the corresponding gene in mice. Absence of α-casein protein significantly curtails secretion of other milk proteins and calcium-phosphate, suggesting a role for α-casein in the establishment of casein micelles. In contrast, secretion of albumin, which is not synthesized in the mammary epithelium, into milk is not reduced. The absence of α-casein also significantly inhibits transcription of the other casein genes. α-Casein deficiency severely delays pup growth during lactation and results in a life-long body size reduction compared to control animals, but has only transient effects on physical and behavioural development of the pups. The data support a critical role for α-casein in casein micelle assembly. The results also confirm lactation as a critical window of metabolic programming and suggest milk protein concentration as a decisive factor in determining adult body weight.
U2 - 10.1371/journal.pone.0021775
DO - 10.1371/journal.pone.0021775
M3 - Journal article
C2 - 21789179
AN - SCOPUS:79960604193
VL - 6
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 7
M1 - e21775
ER -
ID: 230151055